Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS18000

Experiment: Glycerol 20 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS17995 and PFLU_RS18000 overlap by 4 nucleotidesPFLU_RS18000 and PFLU_RS18005 are separated by 30 nucleotides PFLU_RS17995: PFLU_RS17995 - LLM class flavin-dependent oxidoreductase, at 4,083,291 to 4,084,655 _RS17995 PFLU_RS18000: PFLU_RS18000 - AhpC/TSA family protein, at 4,084,652 to 4,085,335 _RS18000 PFLU_RS18005: PFLU_RS18005 - acyl-CoA/acyl-ACP dehydrogenase, at 4,085,366 to 4,086,544 _RS18005 Position (kb) 4084 4085 4086Strain fitness (log2 ratio) -2 -1 0 1at 4083.818 kb on + strand, within PFLU_RS17995at 4083.819 kb on - strand, within PFLU_RS17995at 4083.822 kb on + strand, within PFLU_RS17995at 4083.822 kb on + strand, within PFLU_RS17995at 4083.822 kb on + strand, within PFLU_RS17995at 4083.822 kb on + strand, within PFLU_RS17995at 4083.823 kb on - strand, within PFLU_RS17995at 4083.823 kb on - strand, within PFLU_RS17995at 4084.245 kb on + strand, within PFLU_RS17995at 4084.246 kb on - strand, within PFLU_RS17995at 4084.246 kb on - strand, within PFLU_RS17995at 4084.351 kb on + strand, within PFLU_RS17995at 4084.351 kb on + strand, within PFLU_RS17995at 4084.351 kb on + strand, within PFLU_RS17995at 4084.351 kb on + strand, within PFLU_RS17995at 4084.352 kb on - strand, within PFLU_RS17995at 4084.352 kb on - strand, within PFLU_RS17995at 4084.498 kb on - strand, within PFLU_RS17995at 4084.555 kb on - strandat 4084.555 kb on - strandat 4084.555 kb on - strandat 4084.595 kb on - strandat 4084.781 kb on + strand, within PFLU_RS18000at 4084.781 kb on + strand, within PFLU_RS18000at 4084.782 kb on - strand, within PFLU_RS18000at 4084.782 kb on - strand, within PFLU_RS18000at 4084.934 kb on + strand, within PFLU_RS18000at 4085.330 kb on - strandat 4085.356 kb on + strandat 4085.356 kb on + strandat 4085.357 kb on - strandat 4085.468 kb on + strandat 4085.569 kb on + strand, within PFLU_RS18005at 4085.570 kb on - strand, within PFLU_RS18005at 4085.649 kb on - strand, within PFLU_RS18005at 4085.852 kb on + strand, within PFLU_RS18005at 4085.852 kb on + strand, within PFLU_RS18005

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycerol 20 mM (C)
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4,083,818 + PFLU_RS17995 0.39 +0.8
4,083,819 - PFLU_RS17995 0.39 -0.6
4,083,822 + PFLU_RS17995 0.39 -0.7
4,083,822 + PFLU_RS17995 0.39 +1.4
4,083,822 + PFLU_RS17995 0.39 -0.7
4,083,822 + PFLU_RS17995 0.39 +0.4
4,083,823 - PFLU_RS17995 0.39 +0.0
4,083,823 - PFLU_RS17995 0.39 -0.5
4,084,245 + PFLU_RS17995 0.70 -1.4
4,084,246 - PFLU_RS17995 0.70 -1.7
4,084,246 - PFLU_RS17995 0.70 -0.5
4,084,351 + PFLU_RS17995 0.78 -0.1
4,084,351 + PFLU_RS17995 0.78 -0.1
4,084,351 + PFLU_RS17995 0.78 -0.1
4,084,351 + PFLU_RS17995 0.78 +0.1
4,084,352 - PFLU_RS17995 0.78 -0.0
4,084,352 - PFLU_RS17995 0.78 +1.2
4,084,498 - PFLU_RS17995 0.88 +0.4
4,084,555 - -0.8
4,084,555 - +0.1
4,084,555 - -0.9
4,084,595 - -1.5
4,084,781 + PFLU_RS18000 0.19 -1.8
4,084,781 + PFLU_RS18000 0.19 +0.3
4,084,782 - PFLU_RS18000 0.19 +0.1
4,084,782 - PFLU_RS18000 0.19 +0.1
4,084,934 + PFLU_RS18000 0.41 -1.7
4,085,330 - +1.6
4,085,356 + +0.6
4,085,356 + -1.7
4,085,357 - +0.6
4,085,468 + +0.3
4,085,569 + PFLU_RS18005 0.17 +0.4
4,085,570 - PFLU_RS18005 0.17 +0.6
4,085,649 - PFLU_RS18005 0.24 +0.2
4,085,852 + PFLU_RS18005 0.41 +0.6
4,085,852 + PFLU_RS18005 0.41 -1.2

Or see this region's nucleotide sequence