Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS02275

Experiment: D-Glucose 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS02270 and PFLU_RS02275 are separated by 11 nucleotidesPFLU_RS02275 and PFLU_RS02280 are separated by 383 nucleotides PFLU_RS02270: PFLU_RS02270 - dihydrolipoyllysine-residue acetyltransferase, at 515,559 to 517,208 _RS02270 PFLU_RS02275: PFLU_RS02275 - pyruvate dehydrogenase (acetyl-transferring), homodimeric type, at 517,220 to 519,865 _RS02275 PFLU_RS02280: PFLU_RS02280 - bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase, at 520,249 to 523,188 _RS02280 Position (kb) 517 518 519 520Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 516.359 kb on - strand, within PFLU_RS02270at 516.624 kb on + strand, within PFLU_RS02270at 517.159 kb on - strandat 517.288 kb on + strandat 517.289 kb on - strandat 517.289 kb on - strandat 517.749 kb on + strand, within PFLU_RS02275at 517.750 kb on - strand, within PFLU_RS02275at 517.827 kb on + strand, within PFLU_RS02275at 518.220 kb on + strand, within PFLU_RS02275at 518.220 kb on + strand, within PFLU_RS02275at 518.221 kb on - strand, within PFLU_RS02275at 518.221 kb on - strand, within PFLU_RS02275at 518.275 kb on - strand, within PFLU_RS02275at 518.467 kb on + strand, within PFLU_RS02275at 518.468 kb on - strand, within PFLU_RS02275at 518.784 kb on + strand, within PFLU_RS02275at 518.785 kb on - strand, within PFLU_RS02275at 518.907 kb on + strand, within PFLU_RS02275at 519.273 kb on + strand, within PFLU_RS02275at 519.469 kb on - strand, within PFLU_RS02275at 519.514 kb on - strand, within PFLU_RS02275at 519.531 kb on + strand, within PFLU_RS02275at 519.532 kb on - strand, within PFLU_RS02275at 519.627 kb on - strandat 519.778 kb on - strandat 519.940 kb on + strandat 519.941 kb on - strandat 519.941 kb on - strandat 519.941 kb on - strandat 519.979 kb on - strandat 519.984 kb on + strandat 520.020 kb on + strandat 520.020 kb on + strandat 520.020 kb on + strandat 520.021 kb on - strandat 520.021 kb on - strandat 520.021 kb on - strandat 520.021 kb on - strandat 520.021 kb on - strandat 520.021 kb on - strandat 520.021 kb on - strandat 520.021 kb on - strandat 520.021 kb on - strandat 520.021 kb on - strandat 520.021 kb on - strandat 520.022 kb on + strandat 520.022 kb on + strandat 520.022 kb on + strandat 520.022 kb on + strandat 520.022 kb on + strandat 520.022 kb on + strandat 520.022 kb on + strandat 520.023 kb on - strandat 520.023 kb on - strandat 520.023 kb on - strandat 520.023 kb on - strandat 520.023 kb on - strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.024 kb on + strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.025 kb on - strandat 520.030 kb on + strandat 520.040 kb on - strandat 520.040 kb on - strandat 520.055 kb on + strandat 520.081 kb on - strandat 520.081 kb on - strandat 520.081 kb on - strandat 520.164 kb on - strandat 520.191 kb on + strandat 520.191 kb on + strandat 520.191 kb on + strandat 520.192 kb on - strandat 520.226 kb on + strandat 520.226 kb on + strandat 520.226 kb on + strandat 520.226 kb on + strandat 520.226 kb on + strandat 520.227 kb on - strandat 520.227 kb on - strandat 520.243 kb on + strandat 520.243 kb on + strandat 520.244 kb on - strandat 520.244 kb on - strandat 520.244 kb on - strandat 520.244 kb on - strandat 520.244 kb on - strandat 520.693 kb on + strand, within PFLU_RS02280at 520.694 kb on - strand, within PFLU_RS02280at 520.694 kb on - strand, within PFLU_RS02280at 520.705 kb on + strand, within PFLU_RS02280at 520.705 kb on + strand, within PFLU_RS02280at 520.705 kb on + strand, within PFLU_RS02280at 520.705 kb on + strand, within PFLU_RS02280at 520.706 kb on - strand, within PFLU_RS02280at 520.706 kb on - strand, within PFLU_RS02280at 520.706 kb on - strand, within PFLU_RS02280at 520.706 kb on - strand, within PFLU_RS02280at 520.706 kb on - strand, within PFLU_RS02280at 520.721 kb on + strand, within PFLU_RS02280at 520.721 kb on + strand, within PFLU_RS02280at 520.721 kb on + strand, within PFLU_RS02280at 520.722 kb on - strand, within PFLU_RS02280at 520.722 kb on - strand, within PFLU_RS02280at 520.722 kb on - strand, within PFLU_RS02280at 520.722 kb on - strand, within PFLU_RS02280at 520.722 kb on - strand, within PFLU_RS02280at 520.722 kb on - strand, within PFLU_RS02280at 520.737 kb on + strand, within PFLU_RS02280at 520.843 kb on + strand, within PFLU_RS02280

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose 10 mM (C)
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516,359 - PFLU_RS02270 0.48 -1.3
516,624 + PFLU_RS02270 0.65 -1.6
517,159 - -0.8
517,288 + -1.6
517,289 - -1.6
517,289 - -1.3
517,749 + PFLU_RS02275 0.20 -1.6
517,750 - PFLU_RS02275 0.20 -1.3
517,827 + PFLU_RS02275 0.23 -2.1
518,220 + PFLU_RS02275 0.38 -1.3
518,220 + PFLU_RS02275 0.38 -0.3
518,221 - PFLU_RS02275 0.38 -1.6
518,221 - PFLU_RS02275 0.38 +0.2
518,275 - PFLU_RS02275 0.40 -1.3
518,467 + PFLU_RS02275 0.47 -0.3
518,468 - PFLU_RS02275 0.47 -0.3
518,784 + PFLU_RS02275 0.59 -0.8
518,785 - PFLU_RS02275 0.59 -1.8
518,907 + PFLU_RS02275 0.64 -1.1
519,273 + PFLU_RS02275 0.78 -1.1
519,469 - PFLU_RS02275 0.85 -1.1
519,514 - PFLU_RS02275 0.87 -1.6
519,531 + PFLU_RS02275 0.87 -1.6
519,532 - PFLU_RS02275 0.87 -0.3
519,627 - +1.2
519,778 - -1.1
519,940 + -1.3
519,941 - -0.9
519,941 - -1.8
519,941 - -1.8
519,979 - -1.3
519,984 + -1.3
520,020 + -1.4
520,020 + +0.4
520,020 + -2.6
520,021 - -0.3
520,021 - +0.6
520,021 - +0.0
520,021 - +0.0
520,021 - -0.5
520,021 - +0.1
520,021 - +1.2
520,021 - -0.6
520,021 - -0.1
520,021 - +0.4
520,021 - -1.3
520,022 + -1.9
520,022 + -0.3
520,022 + -2.8
520,022 + -2.0
520,022 + -1.5
520,022 + -1.9
520,022 + -0.3
520,023 - -1.3
520,023 - +1.2
520,023 - +1.2
520,023 - +0.9
520,023 - +0.2
520,024 + -0.5
520,024 + -2.2
520,024 + -1.1
520,024 + -0.3
520,024 + -1.3
520,024 + -2.3
520,024 + -1.3
520,024 + -1.8
520,024 + -1.8
520,024 + -1.8
520,024 + -2.7
520,024 + -0.9
520,024 + -2.3
520,024 + -0.3
520,024 + -0.3
520,025 - -1.3
520,025 - -1.7
520,025 - -0.6
520,025 - +0.2
520,025 - +0.0
520,025 - -0.0
520,025 - +0.5
520,025 - -0.5
520,025 - +0.1
520,025 - -0.5
520,025 - -0.4
520,025 - -0.3
520,025 - -0.0
520,025 - +1.4
520,025 - -2.3
520,025 - +0.6
520,030 + +0.2
520,040 - -0.8
520,040 - -1.3
520,055 + -0.3
520,081 - +0.9
520,081 - -0.3
520,081 - -0.2
520,164 - +1.1
520,191 + +2.7
520,191 + +0.1
520,191 + -1.6
520,192 - -0.5
520,226 + +1.2
520,226 + +0.0
520,226 + +0.2
520,226 + -0.6
520,226 + -0.1
520,227 - -1.9
520,227 - +0.3
520,243 + -0.6
520,243 + -0.5
520,244 - +1.0
520,244 - +0.6
520,244 - +0.2
520,244 - +0.2
520,244 - -0.6
520,693 + PFLU_RS02280 0.15 -0.0
520,694 - PFLU_RS02280 0.15 -0.8
520,694 - PFLU_RS02280 0.15 +1.2
520,705 + PFLU_RS02280 0.16 -0.7
520,705 + PFLU_RS02280 0.16 +0.2
520,705 + PFLU_RS02280 0.16 -0.5
520,705 + PFLU_RS02280 0.16 -0.3
520,706 - PFLU_RS02280 0.16 -0.8
520,706 - PFLU_RS02280 0.16 -0.2
520,706 - PFLU_RS02280 0.16 +0.1
520,706 - PFLU_RS02280 0.16 -0.5
520,706 - PFLU_RS02280 0.16 +0.1
520,721 + PFLU_RS02280 0.16 -0.2
520,721 + PFLU_RS02280 0.16 -1.3
520,721 + PFLU_RS02280 0.16 +0.4
520,722 - PFLU_RS02280 0.16 +0.3
520,722 - PFLU_RS02280 0.16 -1.5
520,722 - PFLU_RS02280 0.16 -1.2
520,722 - PFLU_RS02280 0.16 -0.5
520,722 - PFLU_RS02280 0.16 -1.3
520,722 - PFLU_RS02280 0.16 +0.1
520,737 + PFLU_RS02280 0.17 +0.1
520,843 + PFLU_RS02280 0.20 -0.3

Or see this region's nucleotide sequence