Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15530 and BPHYT_RS15535 are separated by 123 nucleotides BPHYT_RS15535 and BPHYT_RS15540 are separated by 83 nucleotides
BPHYT_RS15530: BPHYT_RS15530 - glutathione ABC transporter permease, at 3,523,216 to 3,524,136
_RS15530
BPHYT_RS15535: BPHYT_RS15535 - glutathione ABC transporter substrate-binding protein GsiB, at 3,524,260 to 3,525,822
_RS15535
BPHYT_RS15540: BPHYT_RS15540 - glutathione ABC transporter ATP-binding protein, at 3,525,906 to 3,527,828
_RS15540
Position (kb)
3524
3525
3526 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.620 kb on - strand, within BPHYT_RS15530 at 3524.052 kb on + strand at 3524.052 kb on + strand at 3524.053 kb on - strand at 3524.053 kb on - strand at 3524.053 kb on - strand at 3524.187 kb on + strand at 3524.309 kb on + strand at 3524.310 kb on - strand at 3524.483 kb on + strand, within BPHYT_RS15535 at 3524.582 kb on + strand, within BPHYT_RS15535 at 3524.586 kb on - strand, within BPHYT_RS15535 at 3524.586 kb on - strand, within BPHYT_RS15535 at 3524.733 kb on - strand, within BPHYT_RS15535 at 3524.738 kb on + strand, within BPHYT_RS15535 at 3524.931 kb on - strand, within BPHYT_RS15535 at 3524.992 kb on - strand, within BPHYT_RS15535 at 3524.993 kb on + strand, within BPHYT_RS15535 at 3524.994 kb on - strand, within BPHYT_RS15535 at 3525.281 kb on + strand, within BPHYT_RS15535 at 3525.281 kb on + strand, within BPHYT_RS15535 at 3525.566 kb on + strand, within BPHYT_RS15535 at 3525.566 kb on + strand, within BPHYT_RS15535 at 3525.626 kb on + strand, within BPHYT_RS15535 at 3525.898 kb on - strand at 3525.910 kb on + strand at 3525.978 kb on - strand at 3526.156 kb on + strand, within BPHYT_RS15540 at 3526.156 kb on + strand, within BPHYT_RS15540 at 3526.156 kb on + strand, within BPHYT_RS15540 at 3526.157 kb on - strand, within BPHYT_RS15540
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 3,523,619 + BPHYT_RS15530 0.44 +0.3 3,523,619 + BPHYT_RS15530 0.44 -0.7 3,523,619 + BPHYT_RS15530 0.44 -0.5 3,523,619 + BPHYT_RS15530 0.44 +0.2 3,523,620 - BPHYT_RS15530 0.44 -1.2 3,524,052 + -0.3 3,524,052 + +0.5 3,524,053 - -0.7 3,524,053 - -1.2 3,524,053 - -0.7 3,524,187 + -1.2 3,524,309 + +0.8 3,524,310 - +0.1 3,524,483 + BPHYT_RS15535 0.14 +0.2 3,524,582 + BPHYT_RS15535 0.21 +1.5 3,524,586 - BPHYT_RS15535 0.21 +2.9 3,524,586 - BPHYT_RS15535 0.21 +0.8 3,524,733 - BPHYT_RS15535 0.30 +0.1 3,524,738 + BPHYT_RS15535 0.31 +1.5 3,524,931 - BPHYT_RS15535 0.43 -1.0 3,524,992 - BPHYT_RS15535 0.47 -1.4 3,524,993 + BPHYT_RS15535 0.47 +1.4 3,524,994 - BPHYT_RS15535 0.47 +1.2 3,525,281 + BPHYT_RS15535 0.65 +0.0 3,525,281 + BPHYT_RS15535 0.65 -0.9 3,525,566 + BPHYT_RS15535 0.84 +0.2 3,525,566 + BPHYT_RS15535 0.84 -0.4 3,525,626 + BPHYT_RS15535 0.87 -0.3 3,525,898 - +1.2 3,525,910 + +0.3 3,525,978 - -1.5 3,526,156 + BPHYT_RS15540 0.13 -0.5 3,526,156 + BPHYT_RS15540 0.13 -0.0 3,526,156 + BPHYT_RS15540 0.13 -0.9 3,526,157 - BPHYT_RS15540 0.13 +0.6
Or see this region's nucleotide sequence