Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15310 and BPHYT_RS15315 are separated by 60 nucleotides BPHYT_RS15315 and BPHYT_RS15320 are separated by 18 nucleotides BPHYT_RS15320 and BPHYT_RS15325 are separated by 210 nucleotides BPHYT_RS15325 and BPHYT_RS15330 overlap by 8 nucleotides
BPHYT_RS15310: BPHYT_RS15310 - DNA-directed RNA polymerase subunit omega, at 3,479,069 to 3,479,272
_RS15310
BPHYT_RS15315: BPHYT_RS15315 - guanylate kinase, at 3,479,333 to 3,480,013
_RS15315
BPHYT_RS15320: BPHYT_RS15320 - hypothetical protein, at 3,480,032 to 3,480,955
_RS15320
BPHYT_RS15325: BPHYT_RS15325 - ribonuclease PH, at 3,481,166 to 3,481,906
_RS15325
BPHYT_RS15330: BPHYT_RS15330 - nucleoside-triphosphate diphosphatase, at 3,481,899 to 3,482,546
_RS15330
Position (kb)
3480
3481 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3479.309 kb on + strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3480.034 kb on - strand at 3480.263 kb on - strand, within BPHYT_RS15320 at 3480.274 kb on - strand, within BPHYT_RS15320 at 3480.274 kb on - strand, within BPHYT_RS15320 at 3480.274 kb on - strand, within BPHYT_RS15320 at 3480.949 kb on - strand at 3480.949 kb on - strand at 3480.959 kb on - strand at 3481.048 kb on - strand at 3481.064 kb on + strand at 3481.065 kb on - strand at 3481.367 kb on + strand, within BPHYT_RS15325 at 3481.652 kb on + strand, within BPHYT_RS15325 at 3481.680 kb on - strand, within BPHYT_RS15325 at 3481.680 kb on - strand, within BPHYT_RS15325 at 3481.858 kb on + strand at 3481.858 kb on + strand at 3481.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 3,479,309 + +1.8 3,479,310 - -0.3 3,479,310 - -0.8 3,479,310 - +0.1 3,479,310 - -0.5 3,479,310 - +0.8 3,479,310 - -0.0 3,479,310 - -0.1 3,480,034 - -0.5 3,480,263 - BPHYT_RS15320 0.25 -0.5 3,480,274 - BPHYT_RS15320 0.26 -1.2 3,480,274 - BPHYT_RS15320 0.26 -2.9 3,480,274 - BPHYT_RS15320 0.26 -0.5 3,480,949 - -0.5 3,480,949 - +0.0 3,480,959 - -1.0 3,481,048 - -0.2 3,481,064 + -0.4 3,481,065 - -0.9 3,481,367 + BPHYT_RS15325 0.27 +0.1 3,481,652 + BPHYT_RS15325 0.66 -1.2 3,481,680 - BPHYT_RS15325 0.69 -1.1 3,481,680 - BPHYT_RS15325 0.69 +1.0 3,481,858 + -0.4 3,481,858 + -0.3 3,481,859 - -2.3
Or see this region's nucleotide sequence