Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS02130

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS02115 and BPHYT_RS02120 overlap by 4 nucleotidesBPHYT_RS02120 and BPHYT_RS02125 are separated by 19 nucleotidesBPHYT_RS02125 and BPHYT_RS02130 are separated by 54 nucleotidesBPHYT_RS02130 and BPHYT_RS02135 are separated by 60 nucleotides BPHYT_RS02115: BPHYT_RS02115 - acyl dehydratase, at 486,833 to 487,324 _RS02115 BPHYT_RS02120: BPHYT_RS02120 - hypothetical protein, at 487,321 to 487,545 _RS02120 BPHYT_RS02125: BPHYT_RS02125 - prolyl-tRNA synthetase, at 487,565 to 488,086 _RS02125 BPHYT_RS02130: BPHYT_RS02130 - hydroxymethylglutaryl-CoA lyase, at 488,141 to 489,067 _RS02130 BPHYT_RS02135: BPHYT_RS02135 - glyoxylate reductase, at 489,128 to 490,069 _RS02135 Position (kb) 488 489 490Strain fitness (log2 ratio) -2 -1 0 1 2at 487.440 kb on + strand, within BPHYT_RS02120at 487.441 kb on - strand, within BPHYT_RS02120at 488.299 kb on - strand, within BPHYT_RS02130at 488.299 kb on - strand, within BPHYT_RS02130at 489.079 kb on + strandat 489.080 kb on - strandat 489.124 kb on - strandat 489.405 kb on + strand, within BPHYT_RS02135at 489.405 kb on + strand, within BPHYT_RS02135at 489.763 kb on - strand, within BPHYT_RS02135at 489.818 kb on - strand, within BPHYT_RS02135

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
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487,440 + BPHYT_RS02120 0.53 -0.2
487,441 - BPHYT_RS02120 0.53 +0.2
488,299 - BPHYT_RS02130 0.17 -1.7
488,299 - BPHYT_RS02130 0.17 -1.9
489,079 + +1.0
489,080 - +0.4
489,124 - -0.1
489,405 + BPHYT_RS02135 0.29 -0.3
489,405 + BPHYT_RS02135 0.29 +0.0
489,763 - BPHYT_RS02135 0.67 -0.2
489,818 - BPHYT_RS02135 0.73 +1.8

Or see this region's nucleotide sequence