Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22030

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22025 and BPHYT_RS22030 are separated by 176 nucleotidesBPHYT_RS22030 and BPHYT_RS22035 overlap by 4 nucleotidesBPHYT_RS22035 and BPHYT_RS22040 are separated by 1 nucleotides BPHYT_RS22025: BPHYT_RS22025 - porin, at 496,195 to 497,784 _RS22025 BPHYT_RS22030: BPHYT_RS22030 - peptide ABC transporter ATP-binding protein, at 497,961 to 498,986 _RS22030 BPHYT_RS22035: BPHYT_RS22035 - peptide ABC transporter ATP-binding protein, at 498,983 to 499,981 _RS22035 BPHYT_RS22040: BPHYT_RS22040 - peptide ABC transporter permease, at 499,983 to 500,897 _RS22040 Position (kb) 497 498 499Strain fitness (log2 ratio) -1 0 1 2 3at 497.109 kb on - strand, within BPHYT_RS22025at 497.148 kb on - strand, within BPHYT_RS22025at 497.342 kb on + strand, within BPHYT_RS22025at 497.375 kb on + strand, within BPHYT_RS22025at 497.640 kb on + strandat 497.681 kb on + strandat 497.789 kb on + strandat 497.963 kb on - strandat 498.200 kb on - strand, within BPHYT_RS22030at 498.221 kb on - strand, within BPHYT_RS22030at 498.263 kb on - strand, within BPHYT_RS22030at 498.693 kb on - strand, within BPHYT_RS22030at 498.986 kb on + strandat 498.986 kb on + strandat 498.986 kb on + strandat 498.987 kb on - strandat 498.987 kb on - strandat 498.987 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
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497,109 - BPHYT_RS22025 0.57 +0.2
497,148 - BPHYT_RS22025 0.60 -0.2
497,342 + BPHYT_RS22025 0.72 +0.7
497,375 + BPHYT_RS22025 0.74 +1.0
497,640 + -0.6
497,681 + -0.1
497,789 + -0.7
497,963 - +1.7
498,200 - BPHYT_RS22030 0.23 +1.3
498,221 - BPHYT_RS22030 0.25 +2.5
498,263 - BPHYT_RS22030 0.29 +1.1
498,693 - BPHYT_RS22030 0.71 +3.5
498,986 + +0.4
498,986 + +0.4
498,986 + -0.8
498,987 - +0.3
498,987 - +0.5
498,987 - +0.8

Or see this region's nucleotide sequence