Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10120

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10115 and BPHYT_RS10120 are separated by 275 nucleotidesBPHYT_RS10120 and BPHYT_RS10125 are separated by 160 nucleotides BPHYT_RS10115: BPHYT_RS10115 - hypothetical protein, at 2,304,178 to 2,304,921 _RS10115 BPHYT_RS10120: BPHYT_RS10120 - membrane protein, at 2,305,197 to 2,305,748 _RS10120 BPHYT_RS10125: BPHYT_RS10125 - hypothetical protein, at 2,305,909 to 2,306,361 _RS10125 Position (kb) 2305 2306Strain fitness (log2 ratio) -1 0 1at 2304.676 kb on + strand, within BPHYT_RS10115at 2304.765 kb on + strand, within BPHYT_RS10115at 2304.852 kb on + strandat 2304.852 kb on + strandat 2304.852 kb on + strandat 2304.864 kb on + strandat 2304.998 kb on + strandat 2304.998 kb on + strandat 2304.999 kb on - strandat 2305.102 kb on + strandat 2305.102 kb on + strandat 2305.196 kb on + strandat 2305.261 kb on + strand, within BPHYT_RS10120at 2305.262 kb on - strand, within BPHYT_RS10120at 2305.262 kb on - strand, within BPHYT_RS10120at 2305.262 kb on - strand, within BPHYT_RS10120at 2305.806 kb on + strandat 2306.194 kb on + strand, within BPHYT_RS10125at 2306.478 kb on - strandat 2306.478 kb on - strandat 2306.702 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
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2,304,676 + BPHYT_RS10115 0.67 +0.3
2,304,765 + BPHYT_RS10115 0.79 +0.6
2,304,852 + +0.3
2,304,852 + +0.6
2,304,852 + -0.5
2,304,864 + +0.0
2,304,998 + +0.9
2,304,998 + -0.1
2,304,999 - -1.1
2,305,102 + -0.2
2,305,102 + +0.4
2,305,196 + -0.5
2,305,261 + BPHYT_RS10120 0.12 -0.9
2,305,262 - BPHYT_RS10120 0.12 +0.0
2,305,262 - BPHYT_RS10120 0.12 -1.1
2,305,262 - BPHYT_RS10120 0.12 -1.3
2,305,806 + -0.1
2,306,194 + BPHYT_RS10125 0.63 -0.9
2,306,478 - -0.3
2,306,478 - +0.2
2,306,702 - +1.1

Or see this region's nucleotide sequence