Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28890 and BPHYT_RS28895 are separated by 21 nucleotides BPHYT_RS28895 and BPHYT_RS28900 are separated by 20 nucleotides BPHYT_RS28900 and BPHYT_RS28905 are separated by 21 nucleotides BPHYT_RS28905 and BPHYT_RS28910 are separated by 52 nucleotides
BPHYT_RS28890: BPHYT_RS28890 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 2,031,050 to 2,031,913
_RS28890
BPHYT_RS28895: BPHYT_RS28895 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 2,031,935 to 2,032,336
_RS28895
BPHYT_RS28900: BPHYT_RS28900 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 2,032,357 to 2,033,160
_RS28900
BPHYT_RS28905: BPHYT_RS28905 - alpha-dehydro-beta-deoxy-D-glucarate aldolase, at 2,033,182 to 2,033,985
_RS28905
BPHYT_RS28910: BPHYT_RS28910 - diguanylate phosphodiesterase, at 2,034,038 to 2,036,371
_RS28910
Position (kb)
2032
2033
2034 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2031.449 kb on - strand, within BPHYT_RS28890 at 2031.744 kb on - strand, within BPHYT_RS28890 at 2032.098 kb on - strand, within BPHYT_RS28895 at 2032.753 kb on + strand, within BPHYT_RS28900 at 2033.029 kb on + strand, within BPHYT_RS28900 at 2033.030 kb on - strand, within BPHYT_RS28900 at 2033.436 kb on - strand, within BPHYT_RS28905
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 2,031,449 - BPHYT_RS28890 0.46 +1.0 2,031,744 - BPHYT_RS28890 0.80 +0.4 2,032,098 - BPHYT_RS28895 0.41 +1.8 2,032,753 + BPHYT_RS28900 0.49 -1.9 2,033,029 + BPHYT_RS28900 0.84 +0.1 2,033,030 - BPHYT_RS28900 0.84 -1.4 2,033,436 - BPHYT_RS28905 0.32 -0.9
Or see this region's nucleotide sequence