Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26410 and BPHYT_RS26415 are separated by 155 nucleotides BPHYT_RS26415 and BPHYT_RS26420 are separated by 109 nucleotides BPHYT_RS26420 and BPHYT_RS26425 are separated by 197 nucleotides BPHYT_RS26425 and BPHYT_RS26430 are separated by 75 nucleotides
BPHYT_RS26410: BPHYT_RS26410 - hypothetical protein, at 1,498,666 to 1,499,055
_RS26410
BPHYT_RS26415: BPHYT_RS26415 - glutathione S-transferase, at 1,499,211 to 1,499,873
_RS26415
BPHYT_RS26420: BPHYT_RS26420 - phytochelatin synthase, at 1,499,983 to 1,500,837
_RS26420
BPHYT_RS26425: BPHYT_RS26425 - Fe-S protein, at 1,501,035 to 1,501,199
_RS26425
BPHYT_RS26430: BPHYT_RS26430 - diguanylate cyclase, at 1,501,275 to 1,502,420
_RS26430
Position (kb) 1499
1500
1501 Strain fitness (log2 ratio) -1
0
1
2 at 1499.173 kb on - strand at 1499.232 kb on + strand at 1499.241 kb on + strand at 1499.523 kb on + strand, within BPHYT_RS26415 at 1499.679 kb on + strand, within BPHYT_RS26415 at 1499.788 kb on - strand, within BPHYT_RS26415 at 1500.060 kb on - strand at 1500.096 kb on + strand, within BPHYT_RS26420 at 1500.504 kb on - strand, within BPHYT_RS26420 at 1501.404 kb on - strand, within BPHYT_RS26430 at 1501.795 kb on + strand, within BPHYT_RS26430 at 1501.831 kb on + strand, within BPHYT_RS26430
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 1,499,173 - -1.4 1,499,232 + -0.7 1,499,241 + +2.2 1,499,523 + BPHYT_RS26415 0.47 -0.6 1,499,679 + BPHYT_RS26415 0.71 +0.7 1,499,788 - BPHYT_RS26415 0.87 +0.3 1,500,060 - -0.3 1,500,096 + BPHYT_RS26420 0.13 +1.5 1,500,504 - BPHYT_RS26420 0.61 +0.6 1,501,404 - BPHYT_RS26430 0.11 +0.4 1,501,795 + BPHYT_RS26430 0.45 +0.1 1,501,831 + BPHYT_RS26430 0.49 +0.2
Or see this region's nucleotide sequence