Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00100 and BPHYT_RS00105 are separated by 43 nucleotides BPHYT_RS00105 and BPHYT_RS00110 overlap by 4 nucleotides BPHYT_RS00110 and BPHYT_RS00115 are separated by 5 nucleotides
BPHYT_RS00100: BPHYT_RS00100 - ethanolamine permease, at 29,985 to 31,388
_RS00100
BPHYT_RS00105: BPHYT_RS00105 - ethanolamine ammonia lyase large subunit, at 31,432 to 32,826
_RS00105
BPHYT_RS00110: BPHYT_RS00110 - ethanolamine ammonia-lyase small subunit, at 32,823 to 33,620
_RS00110
BPHYT_RS00115: BPHYT_RS00115 - acetaldehyde dehydrogenase, at 33,626 to 34,021
_RS00115
Position (kb) 31
32
33 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 30.826 kb on - strand, within BPHYT_RS00100 at 30.857 kb on + strand, within BPHYT_RS00100 at 30.857 kb on + strand, within BPHYT_RS00100 at 30.858 kb on - strand, within BPHYT_RS00100 at 31.047 kb on + strand, within BPHYT_RS00100 at 31.131 kb on + strand, within BPHYT_RS00100 at 31.132 kb on - strand, within BPHYT_RS00100 at 31.132 kb on - strand, within BPHYT_RS00100 at 31.133 kb on + strand, within BPHYT_RS00100 at 31.134 kb on - strand, within BPHYT_RS00100 at 31.439 kb on - strand at 31.768 kb on - strand, within BPHYT_RS00105 at 31.979 kb on - strand, within BPHYT_RS00105 at 32.236 kb on + strand, within BPHYT_RS00105 at 32.237 kb on - strand, within BPHYT_RS00105 at 32.287 kb on + strand, within BPHYT_RS00105 at 32.288 kb on - strand, within BPHYT_RS00105 at 32.288 kb on - strand, within BPHYT_RS00105 at 32.360 kb on + strand, within BPHYT_RS00105 at 32.361 kb on - strand, within BPHYT_RS00105 at 32.368 kb on + strand, within BPHYT_RS00105 at 32.434 kb on + strand, within BPHYT_RS00105 at 32.441 kb on - strand, within BPHYT_RS00105 at 32.598 kb on + strand, within BPHYT_RS00105 at 32.644 kb on + strand, within BPHYT_RS00105 at 32.644 kb on + strand, within BPHYT_RS00105 at 32.907 kb on - strand, within BPHYT_RS00110 at 33.393 kb on + strand, within BPHYT_RS00110 at 33.492 kb on + strand, within BPHYT_RS00110 at 33.618 kb on + strand at 33.710 kb on + strand, within BPHYT_RS00115 at 33.813 kb on + strand, within BPHYT_RS00115
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 30,826 - BPHYT_RS00100 0.60 -1.3 30,857 + BPHYT_RS00100 0.62 +1.3 30,857 + BPHYT_RS00100 0.62 -0.8 30,858 - BPHYT_RS00100 0.62 -3.6 31,047 + BPHYT_RS00100 0.76 -0.6 31,131 + BPHYT_RS00100 0.82 -0.3 31,132 - BPHYT_RS00100 0.82 -1.1 31,132 - BPHYT_RS00100 0.82 +0.2 31,133 + BPHYT_RS00100 0.82 +0.6 31,134 - BPHYT_RS00100 0.82 +0.1 31,439 - +2.2 31,768 - BPHYT_RS00105 0.24 +0.1 31,979 - BPHYT_RS00105 0.39 -2.4 32,236 + BPHYT_RS00105 0.58 -0.4 32,237 - BPHYT_RS00105 0.58 -1.3 32,287 + BPHYT_RS00105 0.61 +0.0 32,288 - BPHYT_RS00105 0.61 -0.6 32,288 - BPHYT_RS00105 0.61 +0.6 32,360 + BPHYT_RS00105 0.67 +1.5 32,361 - BPHYT_RS00105 0.67 -1.9 32,368 + BPHYT_RS00105 0.67 -1.1 32,434 + BPHYT_RS00105 0.72 -1.1 32,441 - BPHYT_RS00105 0.72 +0.7 32,598 + BPHYT_RS00105 0.84 +0.2 32,644 + BPHYT_RS00105 0.87 +0.3 32,644 + BPHYT_RS00105 0.87 +0.1 32,907 - BPHYT_RS00110 0.11 +0.6 33,393 + BPHYT_RS00110 0.71 +0.7 33,492 + BPHYT_RS00110 0.84 -1.4 33,618 + +0.5 33,710 + BPHYT_RS00115 0.21 -1.1 33,813 + BPHYT_RS00115 0.47 -1.2
Or see this region's nucleotide sequence