Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32865

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32855 and BPHYT_RS32860 are separated by 0 nucleotidesBPHYT_RS32860 and BPHYT_RS32865 are separated by 307 nucleotidesBPHYT_RS32865 and BPHYT_RS32870 are separated by 207 nucleotidesBPHYT_RS32870 and BPHYT_RS32875 are separated by 206 nucleotides BPHYT_RS32855: BPHYT_RS32855 - TetR family transcriptional regulator, at 2,951,980 to 2,952,474 _RS32855 BPHYT_RS32860: BPHYT_RS32860 - hypothetical protein, at 2,952,475 to 2,952,702 _RS32860 BPHYT_RS32865: BPHYT_RS32865 - choloylglycine hydrolase, at 2,953,010 to 2,954,089 _RS32865 BPHYT_RS32870: BPHYT_RS32870 - hypothetical protein, at 2,954,297 to 2,954,743 _RS32870 BPHYT_RS32875: BPHYT_RS32875 - GCN5 family acetyltransferase, at 2,954,950 to 2,955,762 _RS32875 Position (kb) 2953 2954 2955Strain fitness (log2 ratio) -1 0 1at 2952.635 kb on - strand, within BPHYT_RS32860at 2952.889 kb on - strandat 2952.999 kb on - strandat 2953.104 kb on + strandat 2953.107 kb on - strandat 2953.731 kb on - strand, within BPHYT_RS32865at 2953.734 kb on + strand, within BPHYT_RS32865at 2953.823 kb on - strand, within BPHYT_RS32865at 2954.006 kb on - strandat 2954.414 kb on + strand, within BPHYT_RS32870at 2954.524 kb on - strand, within BPHYT_RS32870at 2954.660 kb on + strand, within BPHYT_RS32870at 2954.773 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
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2,952,635 - BPHYT_RS32860 0.70 -0.1
2,952,889 - -1.3
2,952,999 - +1.3
2,953,104 + +0.7
2,953,107 - -0.8
2,953,731 - BPHYT_RS32865 0.67 -0.1
2,953,734 + BPHYT_RS32865 0.67 +0.3
2,953,823 - BPHYT_RS32865 0.75 -0.1
2,954,006 - +1.2
2,954,414 + BPHYT_RS32870 0.26 +1.0
2,954,524 - BPHYT_RS32870 0.51 +0.0
2,954,660 + BPHYT_RS32870 0.81 -0.8
2,954,773 + +0.1

Or see this region's nucleotide sequence