Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22090

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22080 and BPHYT_RS22085 are separated by 12 nucleotidesBPHYT_RS22085 and BPHYT_RS22090 are separated by 173 nucleotidesBPHYT_RS22090 and BPHYT_RS22095 are separated by 224 nucleotides BPHYT_RS22080: BPHYT_RS22080 - hypothetical protein, at 511,329 to 512,279 _RS22080 BPHYT_RS22085: BPHYT_RS22085 - transglutaminase, at 512,292 to 513,092 _RS22085 BPHYT_RS22090: BPHYT_RS22090 - hypothetical protein, at 513,266 to 514,138 _RS22090 BPHYT_RS22095: BPHYT_RS22095 - porin, at 514,363 to 515,544 _RS22095 Position (kb) 513 514 515Strain fitness (log2 ratio) -2 -1 0 1 2 3at 512.292 kb on - strandat 512.292 kb on - strandat 512.295 kb on + strandat 512.296 kb on - strandat 512.296 kb on - strandat 512.323 kb on - strandat 512.715 kb on + strand, within BPHYT_RS22085at 512.716 kb on - strand, within BPHYT_RS22085at 512.741 kb on + strand, within BPHYT_RS22085at 512.742 kb on - strand, within BPHYT_RS22085at 512.742 kb on - strand, within BPHYT_RS22085at 512.780 kb on - strand, within BPHYT_RS22085at 512.842 kb on - strand, within BPHYT_RS22085at 512.853 kb on + strand, within BPHYT_RS22085at 512.854 kb on - strand, within BPHYT_RS22085at 512.997 kb on + strand, within BPHYT_RS22085at 513.121 kb on + strandat 513.121 kb on + strandat 513.122 kb on - strandat 513.122 kb on - strandat 513.122 kb on - strandat 513.149 kb on + strandat 513.191 kb on + strandat 513.191 kb on + strandat 513.191 kb on + strandat 513.194 kb on - strandat 513.194 kb on - strandat 513.194 kb on - strandat 513.265 kb on + strandat 513.272 kb on + strandat 513.272 kb on + strandat 513.272 kb on + strandat 513.272 kb on + strandat 513.273 kb on - strandat 513.273 kb on - strandat 513.394 kb on + strand, within BPHYT_RS22090at 513.395 kb on - strand, within BPHYT_RS22090at 513.609 kb on + strand, within BPHYT_RS22090at 513.654 kb on - strand, within BPHYT_RS22090at 513.696 kb on - strand, within BPHYT_RS22090at 513.855 kb on - strand, within BPHYT_RS22090at 513.855 kb on - strand, within BPHYT_RS22090at 513.878 kb on - strand, within BPHYT_RS22090at 513.911 kb on + strand, within BPHYT_RS22090at 513.911 kb on + strand, within BPHYT_RS22090at 514.136 kb on + strandat 514.422 kb on - strandat 514.428 kb on - strandat 514.474 kb on - strandat 514.474 kb on - strandat 514.515 kb on - strand, within BPHYT_RS22095at 514.541 kb on + strand, within BPHYT_RS22095at 514.568 kb on + strand, within BPHYT_RS22095at 514.602 kb on - strand, within BPHYT_RS22095at 514.602 kb on - strand, within BPHYT_RS22095at 514.607 kb on + strand, within BPHYT_RS22095at 514.607 kb on + strand, within BPHYT_RS22095at 514.607 kb on + strand, within BPHYT_RS22095at 514.608 kb on - strand, within BPHYT_RS22095at 514.608 kb on - strand, within BPHYT_RS22095at 514.652 kb on + strand, within BPHYT_RS22095at 514.670 kb on + strand, within BPHYT_RS22095at 514.671 kb on - strand, within BPHYT_RS22095at 514.713 kb on - strand, within BPHYT_RS22095at 514.760 kb on + strand, within BPHYT_RS22095at 514.806 kb on - strand, within BPHYT_RS22095at 514.917 kb on - strand, within BPHYT_RS22095at 514.949 kb on + strand, within BPHYT_RS22095at 514.950 kb on - strand, within BPHYT_RS22095at 515.012 kb on + strand, within BPHYT_RS22095at 515.012 kb on + strandat 515.013 kb on - strand, within BPHYT_RS22095at 515.013 kb on - strand, within BPHYT_RS22095at 515.013 kb on - strand, within BPHYT_RS22095at 515.054 kb on + strand, within BPHYT_RS22095at 515.055 kb on - strand, within BPHYT_RS22095

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
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512,292 - -0.1
512,292 - +0.0
512,295 + -0.3
512,296 - +0.5
512,296 - +0.3
512,323 - +1.3
512,715 + BPHYT_RS22085 0.53 -1.1
512,716 - BPHYT_RS22085 0.53 -2.0
512,741 + BPHYT_RS22085 0.56 -0.2
512,742 - BPHYT_RS22085 0.56 -0.4
512,742 - BPHYT_RS22085 0.56 -0.2
512,780 - BPHYT_RS22085 0.61 -0.0
512,842 - BPHYT_RS22085 0.69 -1.9
512,853 + BPHYT_RS22085 0.70 -0.0
512,854 - BPHYT_RS22085 0.70 +0.1
512,997 + BPHYT_RS22085 0.88 +0.8
513,121 + +0.5
513,121 + -0.8
513,122 - -0.1
513,122 - +0.0
513,122 - +0.3
513,149 + -0.1
513,191 + -0.6
513,191 + +0.2
513,191 + +2.5
513,194 - -0.4
513,194 - -1.8
513,194 - -0.4
513,265 + +0.5
513,272 + +0.3
513,272 + -1.1
513,272 + +0.4
513,272 + -0.2
513,273 - +0.4
513,273 - -2.1
513,394 + BPHYT_RS22090 0.15 +0.6
513,395 - BPHYT_RS22090 0.15 +0.4
513,609 + BPHYT_RS22090 0.39 +0.5
513,654 - BPHYT_RS22090 0.44 +0.8
513,696 - BPHYT_RS22090 0.49 +0.2
513,855 - BPHYT_RS22090 0.67 +0.3
513,855 - BPHYT_RS22090 0.67 -1.3
513,878 - BPHYT_RS22090 0.70 +0.3
513,911 + BPHYT_RS22090 0.74 +0.1
513,911 + BPHYT_RS22090 0.74 -1.0
514,136 + -0.1
514,422 - +0.5
514,428 - -0.4
514,474 - -0.5
514,474 - -1.0
514,515 - BPHYT_RS22095 0.13 -1.6
514,541 + BPHYT_RS22095 0.15 +0.9
514,568 + BPHYT_RS22095 0.17 +0.4
514,602 - BPHYT_RS22095 0.20 -0.8
514,602 - BPHYT_RS22095 0.20 -0.6
514,607 + BPHYT_RS22095 0.21 +1.0
514,607 + BPHYT_RS22095 0.21 +0.3
514,607 + BPHYT_RS22095 0.21 -0.6
514,608 - BPHYT_RS22095 0.21 -0.5
514,608 - BPHYT_RS22095 0.21 +0.7
514,652 + BPHYT_RS22095 0.24 -0.4
514,670 + BPHYT_RS22095 0.26 +3.6
514,671 - BPHYT_RS22095 0.26 +0.3
514,713 - BPHYT_RS22095 0.30 +1.1
514,760 + BPHYT_RS22095 0.34 -0.2
514,806 - BPHYT_RS22095 0.37 -1.6
514,917 - BPHYT_RS22095 0.47 -0.7
514,949 + BPHYT_RS22095 0.50 -1.0
514,950 - BPHYT_RS22095 0.50 -0.4
515,012 + BPHYT_RS22095 0.55 +0.2
515,012 + +1.7
515,013 - BPHYT_RS22095 0.55 -0.2
515,013 - BPHYT_RS22095 0.55 -1.2
515,013 - BPHYT_RS22095 0.55 +1.3
515,054 + BPHYT_RS22095 0.58 +0.3
515,055 - BPHYT_RS22095 0.59 +0.6

Or see this region's nucleotide sequence