Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31385

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31375 and BPHYT_RS31380 are separated by 102 nucleotidesBPHYT_RS31380 and BPHYT_RS31385 are separated by 63 nucleotidesBPHYT_RS31385 and BPHYT_RS31390 overlap by 7 nucleotidesBPHYT_RS31390 and BPHYT_RS31395 are separated by 148 nucleotides BPHYT_RS31375: BPHYT_RS31375 - AraC family transcriptional regulator, at 2,593,776 to 2,594,693 _RS31375 BPHYT_RS31380: BPHYT_RS31380 - dehydrogenase, at 2,594,796 to 2,595,545 _RS31380 BPHYT_RS31385: BPHYT_RS31385 - ketoacyl reductase, at 2,595,609 to 2,596,367 _RS31385 BPHYT_RS31390: BPHYT_RS31390 - hypothetical protein, at 2,596,361 to 2,596,591 _RS31390 BPHYT_RS31395: BPHYT_RS31395 - thiamine pyrophosphate-binding protein, at 2,596,740 to 2,598,488 _RS31395 Position (kb) 2595 2596 2597Strain fitness (log2 ratio) -1 0 1 2at 2594.806 kb on + strandat 2595.574 kb on + strandat 2595.591 kb on + strandat 2595.635 kb on - strandat 2596.128 kb on + strand, within BPHYT_RS31385at 2596.249 kb on - strand, within BPHYT_RS31385at 2596.483 kb on + strand, within BPHYT_RS31390at 2596.484 kb on - strand, within BPHYT_RS31390at 2596.549 kb on + strand, within BPHYT_RS31390at 2596.800 kb on + strandat 2596.800 kb on + strandat 2597.032 kb on + strand, within BPHYT_RS31395at 2597.183 kb on - strand, within BPHYT_RS31395

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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2,594,806 + -0.1
2,595,574 + +0.0
2,595,591 + +0.9
2,595,635 - -0.2
2,596,128 + BPHYT_RS31385 0.68 -1.4
2,596,249 - BPHYT_RS31385 0.84 -1.2
2,596,483 + BPHYT_RS31390 0.53 -0.4
2,596,484 - BPHYT_RS31390 0.53 +0.5
2,596,549 + BPHYT_RS31390 0.81 +1.9
2,596,800 + +0.5
2,596,800 + +0.6
2,597,032 + BPHYT_RS31395 0.17 -0.8
2,597,183 - BPHYT_RS31395 0.25 +0.8

Or see this region's nucleotide sequence