Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31310 and BPHYT_RS31315 are separated by 245 nucleotides BPHYT_RS31315 and BPHYT_RS31320 are separated by 71 nucleotides BPHYT_RS31320 and BPHYT_RS31325 are separated by 37 nucleotides BPHYT_RS31325 and BPHYT_RS31330 are separated by 60 nucleotides BPHYT_RS31330 and BPHYT_RS31335 are separated by 20 nucleotides
BPHYT_RS31310: BPHYT_RS31310 - hypothetical protein, at 2,583,329 to 2,583,604
_RS31310
BPHYT_RS31315: BPHYT_RS31315 - cytochrome C, at 2,583,850 to 2,584,185
_RS31315
BPHYT_RS31320: BPHYT_RS31320 - hypothetical protein, at 2,584,257 to 2,584,631
_RS31320
BPHYT_RS31325: BPHYT_RS31325 - hypothetical protein, at 2,584,669 to 2,585,100
_RS31325
BPHYT_RS31330: BPHYT_RS31330 - MerR family transcriptional regulator, at 2,585,161 to 2,585,466
_RS31330
BPHYT_RS31335: BPHYT_RS31335 - cytochrome C biogenesis protein, at 2,585,487 to 2,586,428
_RS31335
Position (kb) 2584
2585 Strain fitness (log2 ratio) -1
0
1 at 2583.734 kb on - strand at 2584.195 kb on + strand at 2584.220 kb on + strand at 2584.412 kb on + strand, within BPHYT_RS31320 at 2585.121 kb on - strand at 2585.456 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 2,583,734 - -0.1 2,584,195 + -0.2 2,584,220 + -1.5 2,584,412 + BPHYT_RS31320 0.41 -1.7 2,585,121 - +0.9 2,585,456 + -0.4
Or see this region's nucleotide sequence