Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02855 and BPHYT_RS02860 are separated by 78 nucleotides BPHYT_RS02860 and BPHYT_RS02865 overlap by 1 nucleotides BPHYT_RS02865 and BPHYT_RS02870 are separated by 87 nucleotides BPHYT_RS02870 and BPHYT_RS02875 are separated by 73 nucleotides
BPHYT_RS02855: BPHYT_RS02855 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 636,794 to 637,675
_RS02855
BPHYT_RS02860: BPHYT_RS02860 - membrane protein, at 637,754 to 638,386
_RS02860
BPHYT_RS02865: BPHYT_RS02865 - hypothetical protein, at 638,386 to 640,227
_RS02865
BPHYT_RS02870: BPHYT_RS02870 - formamidopyrimidine-DNA glycosylase, at 640,315 to 641,145
_RS02870
BPHYT_RS02875: BPHYT_RS02875 - adenine glycosylase, at 641,219 to 642,280
_RS02875
Position (kb) 638
639
640
641 Strain fitness (log2 ratio) -2
-1
0
1 at 638.164 kb on - strand, within BPHYT_RS02860 at 638.516 kb on - strand at 638.730 kb on - strand, within BPHYT_RS02865 at 638.730 kb on - strand, within BPHYT_RS02865 at 638.823 kb on - strand, within BPHYT_RS02865 at 638.986 kb on - strand, within BPHYT_RS02865 at 639.134 kb on + strand, within BPHYT_RS02865 at 639.791 kb on + strand, within BPHYT_RS02865 at 640.257 kb on + strand at 640.257 kb on + strand at 640.270 kb on - strand at 640.275 kb on - strand at 640.275 kb on - strand at 640.310 kb on + strand at 640.311 kb on - strand at 640.363 kb on + strand at 640.364 kb on - strand at 640.364 kb on - strand at 640.364 kb on - strand at 640.364 kb on - strand at 640.402 kb on - strand, within BPHYT_RS02870 at 640.489 kb on + strand, within BPHYT_RS02870 at 640.489 kb on + strand, within BPHYT_RS02870 at 640.490 kb on - strand at 640.760 kb on + strand, within BPHYT_RS02870 at 640.761 kb on - strand, within BPHYT_RS02870 at 640.842 kb on + strand, within BPHYT_RS02870 at 640.843 kb on - strand, within BPHYT_RS02870 at 640.843 kb on - strand, within BPHYT_RS02870 at 640.843 kb on - strand, within BPHYT_RS02870 at 640.843 kb on - strand, within BPHYT_RS02870 at 640.843 kb on - strand, within BPHYT_RS02870 at 640.846 kb on + strand, within BPHYT_RS02870 at 640.846 kb on + strand, within BPHYT_RS02870 at 640.847 kb on - strand, within BPHYT_RS02870 at 640.847 kb on - strand, within BPHYT_RS02870 at 640.847 kb on - strand, within BPHYT_RS02870 at 641.020 kb on + strand, within BPHYT_RS02870 at 641.035 kb on + strand, within BPHYT_RS02870 at 641.036 kb on - strand, within BPHYT_RS02870 at 641.036 kb on - strand, within BPHYT_RS02870 at 641.119 kb on + strand at 641.119 kb on + strand at 641.120 kb on - strand at 641.144 kb on - strand at 641.144 kb on - strand at 641.166 kb on + strand at 641.166 kb on + strand at 641.167 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 638,164 - BPHYT_RS02860 0.65 -0.4 638,516 - +1.0 638,730 - BPHYT_RS02865 0.19 -0.3 638,730 - BPHYT_RS02865 0.19 -0.6 638,823 - BPHYT_RS02865 0.24 -0.0 638,986 - BPHYT_RS02865 0.33 -0.0 639,134 + BPHYT_RS02865 0.41 +0.4 639,791 + BPHYT_RS02865 0.76 -1.5 640,257 + -0.7 640,257 + +0.4 640,270 - -1.2 640,275 - +1.6 640,275 - +0.1 640,310 + -0.9 640,311 - +0.6 640,363 + -0.3 640,364 - -0.2 640,364 - +0.7 640,364 - +0.4 640,364 - +0.7 640,402 - BPHYT_RS02870 0.10 -0.5 640,489 + BPHYT_RS02870 0.21 +1.4 640,489 + BPHYT_RS02870 0.21 -0.1 640,490 - -0.8 640,760 + BPHYT_RS02870 0.54 -2.1 640,761 - BPHYT_RS02870 0.54 -0.4 640,842 + BPHYT_RS02870 0.63 +1.0 640,843 - BPHYT_RS02870 0.64 +0.4 640,843 - BPHYT_RS02870 0.64 +0.4 640,843 - BPHYT_RS02870 0.64 -0.2 640,843 - BPHYT_RS02870 0.64 -0.8 640,843 - BPHYT_RS02870 0.64 +0.7 640,846 + BPHYT_RS02870 0.64 -0.8 640,846 + BPHYT_RS02870 0.64 +0.6 640,847 - BPHYT_RS02870 0.64 +0.1 640,847 - BPHYT_RS02870 0.64 +1.5 640,847 - BPHYT_RS02870 0.64 +0.0 641,020 + BPHYT_RS02870 0.85 +1.6 641,035 + BPHYT_RS02870 0.87 -1.0 641,036 - BPHYT_RS02870 0.87 -0.9 641,036 - BPHYT_RS02870 0.87 +0.2 641,119 + +0.6 641,119 + +0.1 641,120 - +0.7 641,144 - -0.8 641,144 - +0.9 641,166 + +0.2 641,166 + +1.3 641,167 - -1.0
Or see this region's nucleotide sequence