Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02240 and BPHYT_RS02245 are separated by 85 nucleotides BPHYT_RS02245 and BPHYT_RS02250 are separated by 187 nucleotides BPHYT_RS02250 and BPHYT_RS02255 are separated by 76 nucleotides BPHYT_RS02255 and BPHYT_RS02260 are separated by 3 nucleotides
BPHYT_RS02240: BPHYT_RS02240 - isocitrate dehydrogenase kinase/phosphatase, at 514,158 to 515,996
_RS02240
BPHYT_RS02245: BPHYT_RS02245 - MerR family transcriptional regulator, at 516,082 to 516,486
_RS02245
BPHYT_RS02250: BPHYT_RS02250 - Zn-dependent hydrolase, at 516,674 to 517,747
_RS02250
BPHYT_RS02255: BPHYT_RS02255 - hypothetical protein, at 517,824 to 518,288
_RS02255
BPHYT_RS02260: BPHYT_RS02260 - hypothetical protein, at 518,292 to 518,660
_RS02260
Position (kb) 516
517
518 Strain fitness (log2 ratio) -2
-1
0
1
2 at 515.719 kb on + strand, within BPHYT_RS02240 at 515.720 kb on - strand, within BPHYT_RS02240 at 515.816 kb on - strand at 515.823 kb on - strand at 516.042 kb on - strand at 516.263 kb on + strand, within BPHYT_RS02245 at 516.330 kb on + strand, within BPHYT_RS02245 at 516.357 kb on - strand, within BPHYT_RS02245 at 516.400 kb on + strand, within BPHYT_RS02245 at 516.473 kb on + strand at 516.473 kb on + strand at 516.591 kb on + strand at 517.215 kb on - strand, within BPHYT_RS02250 at 517.416 kb on + strand, within BPHYT_RS02250 at 517.468 kb on - strand, within BPHYT_RS02250 at 517.594 kb on + strand, within BPHYT_RS02250 at 517.822 kb on + strand at 517.825 kb on + strand at 517.825 kb on + strand at 517.826 kb on - strand at 518.003 kb on - strand, within BPHYT_RS02255 at 518.054 kb on + strand, within BPHYT_RS02255 at 518.056 kb on + strand, within BPHYT_RS02255 at 518.057 kb on - strand, within BPHYT_RS02255 at 518.091 kb on + strand, within BPHYT_RS02255 at 518.101 kb on + strand, within BPHYT_RS02255 at 518.101 kb on + strand, within BPHYT_RS02255 at 518.102 kb on - strand, within BPHYT_RS02255 at 518.162 kb on + strand at 518.162 kb on + strand, within BPHYT_RS02255 at 518.165 kb on - strand, within BPHYT_RS02255 at 518.165 kb on - strand, within BPHYT_RS02255 at 518.265 kb on - strand at 518.359 kb on + strand, within BPHYT_RS02260 at 518.360 kb on - strand, within BPHYT_RS02260 at 518.399 kb on - strand, within BPHYT_RS02260 at 518.443 kb on - strand, within BPHYT_RS02260 at 518.479 kb on - strand, within BPHYT_RS02260 at 518.485 kb on + strand, within BPHYT_RS02260 at 518.485 kb on + strand, within BPHYT_RS02260 at 518.515 kb on + strand, within BPHYT_RS02260 at 518.531 kb on - strand, within BPHYT_RS02260 at 518.646 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 515,719 + BPHYT_RS02240 0.85 +0.1 515,720 - BPHYT_RS02240 0.85 +0.0 515,816 - +1.3 515,823 - -1.7 516,042 - -1.8 516,263 + BPHYT_RS02245 0.45 +1.0 516,330 + BPHYT_RS02245 0.61 -0.9 516,357 - BPHYT_RS02245 0.68 -2.0 516,400 + BPHYT_RS02245 0.79 -0.8 516,473 + +0.5 516,473 + -0.9 516,591 + +0.3 517,215 - BPHYT_RS02250 0.50 +1.5 517,416 + BPHYT_RS02250 0.69 +0.3 517,468 - BPHYT_RS02250 0.74 +0.1 517,594 + BPHYT_RS02250 0.86 +0.0 517,822 + -1.3 517,825 + -0.0 517,825 + +1.0 517,826 - -1.0 518,003 - BPHYT_RS02255 0.38 -0.2 518,054 + BPHYT_RS02255 0.49 +1.9 518,056 + BPHYT_RS02255 0.50 +0.3 518,057 - BPHYT_RS02255 0.50 -0.3 518,091 + BPHYT_RS02255 0.57 -0.0 518,101 + BPHYT_RS02255 0.60 +0.7 518,101 + BPHYT_RS02255 0.60 +1.9 518,102 - BPHYT_RS02255 0.60 -0.6 518,162 + -0.8 518,162 + BPHYT_RS02255 0.73 +0.3 518,165 - BPHYT_RS02255 0.73 -0.6 518,165 - BPHYT_RS02255 0.73 +0.3 518,265 - +0.4 518,359 + BPHYT_RS02260 0.18 -0.2 518,360 - BPHYT_RS02260 0.18 +0.6 518,399 - BPHYT_RS02260 0.29 -0.1 518,443 - BPHYT_RS02260 0.41 +0.1 518,479 - BPHYT_RS02260 0.51 -1.2 518,485 + BPHYT_RS02260 0.52 +0.1 518,485 + BPHYT_RS02260 0.52 -0.4 518,515 + BPHYT_RS02260 0.60 +0.4 518,531 - BPHYT_RS02260 0.65 +1.8 518,646 - +1.5
Or see this region's nucleotide sequence