Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12840

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12835 and BPHYT_RS12840 are separated by 87 nucleotidesBPHYT_RS12840 and BPHYT_RS12845 are separated by 275 nucleotides BPHYT_RS12835: BPHYT_RS12835 - dihydrolipoamide acetyltransferase, at 2,924,895 to 2,926,547 _RS12835 BPHYT_RS12840: BPHYT_RS12840 - pyruvate dehydrogenase, at 2,926,635 to 2,929,331 _RS12840 BPHYT_RS12845: BPHYT_RS12845 - ATPase, at 2,929,607 to 2,932,123 _RS12845 Position (kb) 2926 2927 2928 2929 2930Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2925.705 kb on - strand, within BPHYT_RS12835at 2926.637 kb on - strandat 2926.637 kb on - strandat 2927.252 kb on - strand, within BPHYT_RS12840at 2927.586 kb on - strand, within BPHYT_RS12840at 2927.586 kb on - strand, within BPHYT_RS12840at 2927.586 kb on - strand, within BPHYT_RS12840at 2927.674 kb on + strand, within BPHYT_RS12840at 2928.202 kb on + strand, within BPHYT_RS12840at 2928.203 kb on - strand, within BPHYT_RS12840at 2928.680 kb on - strandat 2928.680 kb on - strand, within BPHYT_RS12840at 2928.866 kb on - strand, within BPHYT_RS12840at 2929.130 kb on - strandat 2929.300 kb on + strandat 2929.348 kb on + strandat 2929.349 kb on - strandat 2929.412 kb on + strandat 2929.544 kb on - strandat 2929.578 kb on + strandat 2929.721 kb on - strandat 2929.822 kb on + strandat 2929.822 kb on + strandat 2929.979 kb on + strand, within BPHYT_RS12845at 2929.980 kb on - strand, within BPHYT_RS12845at 2929.980 kb on - strand, within BPHYT_RS12845at 2929.980 kb on - strandat 2929.980 kb on - strand, within BPHYT_RS12845at 2929.980 kb on - strand, within BPHYT_RS12845at 2929.980 kb on - strand, within BPHYT_RS12845at 2929.992 kb on - strand, within BPHYT_RS12845at 2929.992 kb on - strand, within BPHYT_RS12845at 2930.117 kb on - strand, within BPHYT_RS12845at 2930.147 kb on + strand, within BPHYT_RS12845at 2930.147 kb on + strand, within BPHYT_RS12845at 2930.148 kb on - strand, within BPHYT_RS12845at 2930.148 kb on - strand, within BPHYT_RS12845at 2930.169 kb on - strand, within BPHYT_RS12845

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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2,925,705 - BPHYT_RS12835 0.49 +2.6
2,926,637 - +0.9
2,926,637 - -1.4
2,927,252 - BPHYT_RS12840 0.23 -0.4
2,927,586 - BPHYT_RS12840 0.35 +0.9
2,927,586 - BPHYT_RS12840 0.35 -1.3
2,927,586 - BPHYT_RS12840 0.35 -3.5
2,927,674 + BPHYT_RS12840 0.39 -0.4
2,928,202 + BPHYT_RS12840 0.58 +0.3
2,928,203 - BPHYT_RS12840 0.58 -0.3
2,928,680 - -0.9
2,928,680 - BPHYT_RS12840 0.76 -1.1
2,928,866 - BPHYT_RS12840 0.83 +2.5
2,929,130 - -1.3
2,929,300 + -2.3
2,929,348 + -1.3
2,929,349 - -0.2
2,929,412 + +1.1
2,929,544 - -0.2
2,929,578 + -1.3
2,929,721 - -2.7
2,929,822 + -4.3
2,929,822 + -3.3
2,929,979 + BPHYT_RS12845 0.15 -3.4
2,929,980 - BPHYT_RS12845 0.15 -1.1
2,929,980 - BPHYT_RS12845 0.15 -3.1
2,929,980 - +0.9
2,929,980 - BPHYT_RS12845 0.15 -3.0
2,929,980 - BPHYT_RS12845 0.15 -1.0
2,929,980 - BPHYT_RS12845 0.15 -0.4
2,929,992 - BPHYT_RS12845 0.15 -3.4
2,929,992 - BPHYT_RS12845 0.15 -2.1
2,930,117 - BPHYT_RS12845 0.20 -0.9
2,930,147 + BPHYT_RS12845 0.21 -2.0
2,930,147 + BPHYT_RS12845 0.21 -4.0
2,930,148 - BPHYT_RS12845 0.21 -3.5
2,930,148 - BPHYT_RS12845 0.21 -4.4
2,930,169 - BPHYT_RS12845 0.22 -3.8

Or see this region's nucleotide sequence