Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12275

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12270 and BPHYT_RS12275 are separated by 560 nucleotidesBPHYT_RS12275 and BPHYT_RS12280 are separated by 527 nucleotides BPHYT_RS12270: BPHYT_RS12270 - O-sialoglycoprotein endopeptidase, at 2,801,879 to 2,802,709 _RS12270 BPHYT_RS12275: BPHYT_RS12275 - acyl-CoA-binding protein, at 2,803,270 to 2,803,539 _RS12275 BPHYT_RS12280: BPHYT_RS12280 - RNA helicase, at 2,804,067 to 2,805,695 _RS12280 Position (kb) 2803 2804Strain fitness (log2 ratio) -2 -1 0 1at 2802.721 kb on + strandat 2803.086 kb on - strandat 2803.218 kb on + strandat 2803.218 kb on + strandat 2803.435 kb on + strand, within BPHYT_RS12275at 2803.435 kb on + strand, within BPHYT_RS12275at 2803.441 kb on + strand, within BPHYT_RS12275at 2803.441 kb on + strand, within BPHYT_RS12275at 2803.441 kb on + strand, within BPHYT_RS12275at 2803.495 kb on + strand, within BPHYT_RS12275at 2803.705 kb on - strandat 2803.707 kb on + strandat 2803.733 kb on + strandat 2803.734 kb on - strandat 2803.734 kb on - strandat 2804.007 kb on + strandat 2804.042 kb on + strandat 2804.042 kb on + strandat 2804.053 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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2,802,721 + -0.4
2,803,086 - +0.1
2,803,218 + -0.3
2,803,218 + -2.2
2,803,435 + BPHYT_RS12275 0.61 +0.3
2,803,435 + BPHYT_RS12275 0.61 +0.4
2,803,441 + BPHYT_RS12275 0.63 +0.6
2,803,441 + BPHYT_RS12275 0.63 +0.0
2,803,441 + BPHYT_RS12275 0.63 +0.0
2,803,495 + BPHYT_RS12275 0.83 +0.3
2,803,705 - +0.7
2,803,707 + +0.1
2,803,733 + -1.3
2,803,734 - -0.5
2,803,734 - +0.6
2,804,007 + -0.9
2,804,042 + -1.8
2,804,042 + +0.9
2,804,053 + +0.2

Or see this region's nucleotide sequence