Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01615 and BPHYT_RS01620 are separated by 134 nucleotides BPHYT_RS01620 and BPHYT_RS01625 overlap by 4 nucleotides BPHYT_RS01625 and BPHYT_RS01630 are separated by 143 nucleotides
BPHYT_RS01615: BPHYT_RS01615 - hypothetical protein, at 366,358 to 367,995
_RS01615
BPHYT_RS01620: BPHYT_RS01620 - sensor histidine kinase, at 368,130 to 369,440
_RS01620
BPHYT_RS01625: BPHYT_RS01625 - chemotaxis protein CheY, at 369,437 to 369,979
_RS01625
BPHYT_RS01630: BPHYT_RS01630 - ATP-dependent protease ATPase subunit HslU, at 370,123 to 371,469
_RS01630
Position (kb) 368
369
370 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 367.323 kb on + strand, within BPHYT_RS01615 at 367.324 kb on - strand, within BPHYT_RS01615 at 367.324 kb on - strand, within BPHYT_RS01615 at 367.324 kb on - strand, within BPHYT_RS01615 at 367.324 kb on - strand, within BPHYT_RS01615 at 367.421 kb on + strand, within BPHYT_RS01615 at 367.421 kb on + strand, within BPHYT_RS01615 at 367.422 kb on - strand, within BPHYT_RS01615 at 367.422 kb on - strand, within BPHYT_RS01615 at 367.512 kb on - strand, within BPHYT_RS01615 at 367.724 kb on + strand, within BPHYT_RS01615 at 367.990 kb on + strand at 367.991 kb on - strand at 368.090 kb on - strand at 368.100 kb on - strand at 368.365 kb on + strand, within BPHYT_RS01620 at 368.365 kb on + strand, within BPHYT_RS01620 at 368.439 kb on + strand, within BPHYT_RS01620 at 368.439 kb on + strand, within BPHYT_RS01620 at 368.439 kb on + strand, within BPHYT_RS01620 at 368.439 kb on + strand, within BPHYT_RS01620 at 368.439 kb on + strand, within BPHYT_RS01620 at 368.584 kb on - strand, within BPHYT_RS01620 at 368.672 kb on - strand, within BPHYT_RS01620 at 368.672 kb on - strand, within BPHYT_RS01620 at 368.672 kb on - strand, within BPHYT_RS01620 at 368.784 kb on - strand, within BPHYT_RS01620 at 368.847 kb on + strand, within BPHYT_RS01620 at 368.848 kb on - strand, within BPHYT_RS01620 at 368.848 kb on - strand, within BPHYT_RS01620 at 368.848 kb on - strand, within BPHYT_RS01620 at 368.849 kb on + strand, within BPHYT_RS01620 at 368.891 kb on + strand, within BPHYT_RS01620 at 368.891 kb on + strand, within BPHYT_RS01620 at 368.892 kb on - strand, within BPHYT_RS01620 at 368.892 kb on - strand, within BPHYT_RS01620 at 369.053 kb on + strand, within BPHYT_RS01620 at 369.172 kb on + strand, within BPHYT_RS01620 at 369.172 kb on + strand, within BPHYT_RS01620 at 369.173 kb on - strand, within BPHYT_RS01620 at 369.173 kb on - strand, within BPHYT_RS01620 at 369.239 kb on + strand, within BPHYT_RS01620 at 369.239 kb on + strand, within BPHYT_RS01620 at 369.239 kb on + strand, within BPHYT_RS01620 at 369.240 kb on - strand, within BPHYT_RS01620 at 369.267 kb on + strand, within BPHYT_RS01620 at 369.359 kb on + strand at 369.737 kb on + strand, within BPHYT_RS01625 at 369.737 kb on + strand, within BPHYT_RS01625 at 369.865 kb on + strand, within BPHYT_RS01625 at 369.866 kb on - strand, within BPHYT_RS01625 at 369.866 kb on - strand, within BPHYT_RS01625 at 369.866 kb on - strand, within BPHYT_RS01625 at 369.977 kb on + strand at 369.977 kb on + strand at 369.978 kb on - strand at 370.016 kb on - strand at 370.268 kb on + strand, within BPHYT_RS01630 at 370.392 kb on - strand, within BPHYT_RS01630
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 367,323 + BPHYT_RS01615 0.59 +5.8 367,324 - BPHYT_RS01615 0.59 -0.5 367,324 - BPHYT_RS01615 0.59 -0.1 367,324 - BPHYT_RS01615 0.59 -0.7 367,324 - BPHYT_RS01615 0.59 -0.2 367,421 + BPHYT_RS01615 0.65 +1.6 367,421 + BPHYT_RS01615 0.65 +1.7 367,422 - BPHYT_RS01615 0.65 -0.8 367,422 - BPHYT_RS01615 0.65 +0.0 367,512 - BPHYT_RS01615 0.70 -0.0 367,724 + BPHYT_RS01615 0.83 +1.6 367,990 + +0.0 367,991 - +0.3 368,090 - -0.4 368,100 - +0.1 368,365 + BPHYT_RS01620 0.18 +0.3 368,365 + BPHYT_RS01620 0.18 -2.8 368,439 + BPHYT_RS01620 0.24 -4.6 368,439 + BPHYT_RS01620 0.24 -3.0 368,439 + BPHYT_RS01620 0.24 -1.8 368,439 + BPHYT_RS01620 0.24 +1.9 368,439 + BPHYT_RS01620 0.24 -0.4 368,584 - BPHYT_RS01620 0.35 -1.6 368,672 - BPHYT_RS01620 0.41 +1.9 368,672 - BPHYT_RS01620 0.41 +0.9 368,672 - BPHYT_RS01620 0.41 +0.1 368,784 - BPHYT_RS01620 0.50 +0.3 368,847 + BPHYT_RS01620 0.55 -1.1 368,848 - BPHYT_RS01620 0.55 +0.3 368,848 - BPHYT_RS01620 0.55 -0.7 368,848 - BPHYT_RS01620 0.55 +0.9 368,849 + BPHYT_RS01620 0.55 -2.0 368,891 + BPHYT_RS01620 0.58 -3.6 368,891 + BPHYT_RS01620 0.58 -4.0 368,892 - BPHYT_RS01620 0.58 +1.9 368,892 - BPHYT_RS01620 0.58 -0.9 369,053 + BPHYT_RS01620 0.70 -0.7 369,172 + BPHYT_RS01620 0.79 -0.4 369,172 + BPHYT_RS01620 0.79 -1.1 369,173 - BPHYT_RS01620 0.80 -0.1 369,173 - BPHYT_RS01620 0.80 +0.9 369,239 + BPHYT_RS01620 0.85 +0.9 369,239 + BPHYT_RS01620 0.85 +0.3 369,239 + BPHYT_RS01620 0.85 +1.6 369,240 - BPHYT_RS01620 0.85 +1.9 369,267 + BPHYT_RS01620 0.87 +1.9 369,359 + -1.4 369,737 + BPHYT_RS01625 0.55 +1.9 369,737 + BPHYT_RS01625 0.55 +1.9 369,865 + BPHYT_RS01625 0.79 +1.9 369,866 - BPHYT_RS01625 0.79 -0.1 369,866 - BPHYT_RS01625 0.79 +1.9 369,866 - BPHYT_RS01625 0.79 +1.9 369,977 + +0.0 369,977 + +0.5 369,978 - +1.5 370,016 - +0.4 370,268 + BPHYT_RS01630 0.11 -0.9 370,392 - BPHYT_RS01630 0.20 -0.5
Or see this region's nucleotide sequence