Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01830 and BPHYT_RS01835 are separated by 278 nucleotides BPHYT_RS01835 and BPHYT_RS01840 are separated by 41 nucleotides BPHYT_RS01840 and BPHYT_RS01845 are separated by 88 nucleotides
BPHYT_RS01830: BPHYT_RS01830 - LacI family transcriptional regulator, at 415,862 to 416,914
_RS01830
BPHYT_RS01835: BPHYT_RS01835 - aldose epimerase, at 417,193 to 418,233
_RS01835
BPHYT_RS01840: BPHYT_RS01840 - endonuclease, at 418,275 to 419,072
_RS01840
BPHYT_RS01845: BPHYT_RS01845 - hypothetical protein, at 419,161 to 419,700
_RS01845
Position (kb) 417
418
419 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 416.368 kb on + strand, within BPHYT_RS01830 at 416.368 kb on + strand, within BPHYT_RS01830 at 416.368 kb on + strand, within BPHYT_RS01830 at 416.369 kb on - strand, within BPHYT_RS01830 at 416.369 kb on - strand, within BPHYT_RS01830 at 416.369 kb on - strand, within BPHYT_RS01830 at 416.370 kb on + strand, within BPHYT_RS01830 at 416.370 kb on + strand, within BPHYT_RS01830 at 416.371 kb on - strand, within BPHYT_RS01830 at 416.371 kb on - strand, within BPHYT_RS01830 at 416.371 kb on - strand, within BPHYT_RS01830 at 416.371 kb on - strand, within BPHYT_RS01830 at 416.471 kb on - strand at 416.472 kb on + strand, within BPHYT_RS01830 at 416.472 kb on + strand, within BPHYT_RS01830 at 416.472 kb on + strand, within BPHYT_RS01830 at 416.491 kb on + strand, within BPHYT_RS01830 at 416.492 kb on - strand, within BPHYT_RS01830 at 416.536 kb on - strand, within BPHYT_RS01830 at 416.628 kb on + strand, within BPHYT_RS01830 at 416.628 kb on + strand, within BPHYT_RS01830 at 416.656 kb on - strand, within BPHYT_RS01830 at 416.724 kb on + strand, within BPHYT_RS01830 at 416.724 kb on + strand, within BPHYT_RS01830 at 416.810 kb on + strand at 416.810 kb on + strand at 416.811 kb on - strand at 416.811 kb on - strand at 416.811 kb on - strand at 417.014 kb on + strand at 417.015 kb on - strand at 417.017 kb on + strand at 417.017 kb on + strand at 417.018 kb on - strand at 417.018 kb on - strand at 417.018 kb on - strand at 417.194 kb on + strand at 417.194 kb on + strand at 417.194 kb on + strand at 417.406 kb on + strand, within BPHYT_RS01835 at 417.512 kb on - strand, within BPHYT_RS01835 at 417.693 kb on - strand, within BPHYT_RS01835 at 417.823 kb on + strand, within BPHYT_RS01835 at 417.853 kb on + strand, within BPHYT_RS01835 at 418.073 kb on + strand, within BPHYT_RS01835 at 418.231 kb on + strand at 418.232 kb on - strand at 418.276 kb on + strand at 418.298 kb on + strand at 418.300 kb on + strand at 418.300 kb on + strand at 418.300 kb on + strand at 418.300 kb on + strand at 418.301 kb on - strand at 418.301 kb on - strand at 418.301 kb on - strand at 418.997 kb on + strand at 418.998 kb on - strand at 419.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 416,368 + BPHYT_RS01830 0.48 +0.6 416,368 + BPHYT_RS01830 0.48 +0.8 416,368 + BPHYT_RS01830 0.48 -0.7 416,369 - BPHYT_RS01830 0.48 -1.6 416,369 - BPHYT_RS01830 0.48 +0.9 416,369 - BPHYT_RS01830 0.48 -0.9 416,370 + BPHYT_RS01830 0.48 +0.2 416,370 + BPHYT_RS01830 0.48 -0.3 416,371 - BPHYT_RS01830 0.48 -0.0 416,371 - BPHYT_RS01830 0.48 -0.6 416,371 - BPHYT_RS01830 0.48 +0.9 416,371 - BPHYT_RS01830 0.48 -0.9 416,471 - -1.9 416,472 + BPHYT_RS01830 0.58 -0.0 416,472 + BPHYT_RS01830 0.58 -0.2 416,472 + BPHYT_RS01830 0.58 -0.7 416,491 + BPHYT_RS01830 0.60 -0.3 416,492 - BPHYT_RS01830 0.60 -0.5 416,536 - BPHYT_RS01830 0.64 -1.6 416,628 + BPHYT_RS01830 0.73 -0.7 416,628 + BPHYT_RS01830 0.73 -1.0 416,656 - BPHYT_RS01830 0.75 +0.5 416,724 + BPHYT_RS01830 0.82 -0.2 416,724 + BPHYT_RS01830 0.82 -1.0 416,810 + -0.2 416,810 + -0.2 416,811 - +0.4 416,811 - +2.1 416,811 - -2.2 417,014 + +1.4 417,015 - -0.5 417,017 + +0.5 417,017 + -0.7 417,018 - +0.1 417,018 - -0.5 417,018 - -0.3 417,194 + +0.7 417,194 + -0.4 417,194 + -0.2 417,406 + BPHYT_RS01835 0.20 -0.7 417,512 - BPHYT_RS01835 0.31 +0.3 417,693 - BPHYT_RS01835 0.48 +0.4 417,823 + BPHYT_RS01835 0.61 -0.8 417,853 + BPHYT_RS01835 0.63 +0.7 418,073 + BPHYT_RS01835 0.85 +0.8 418,231 + -0.6 418,232 - -0.1 418,276 + -0.6 418,298 + -0.8 418,300 + -1.8 418,300 + -1.2 418,300 + +0.2 418,300 + -1.9 418,301 - -3.5 418,301 - -2.0 418,301 - -0.1 418,997 + +1.1 418,998 - -2.9 419,038 - +0.3
Or see this region's nucleotide sequence