Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11220 and BPHYT_RS11225 are separated by 361 nucleotides BPHYT_RS11225 and BPHYT_RS11230 are separated by 208 nucleotides BPHYT_RS11230 and BPHYT_RS11235 are separated by 136 nucleotides
BPHYT_RS11220: BPHYT_RS11220 - LacI family transcriptional regulator, at 2,552,694 to 2,553,656
_RS11220
BPHYT_RS11225: BPHYT_RS11225 - magnesium transporter, at 2,554,018 to 2,554,995
_RS11225
BPHYT_RS11230: BPHYT_RS11230 - hypothetical protein, at 2,555,204 to 2,555,680
_RS11230
BPHYT_RS11235: BPHYT_RS11235 - hypothetical protein, at 2,555,817 to 2,556,050
_RS11235
Position (kb)
2554
2555 Strain fitness (log2 ratio)
-1
0
1 at 2553.117 kb on - strand, within BPHYT_RS11220 at 2553.178 kb on + strand, within BPHYT_RS11220 at 2553.476 kb on - strand, within BPHYT_RS11220 at 2553.661 kb on - strand at 2553.661 kb on - strand at 2553.661 kb on - strand at 2553.661 kb on - strand at 2553.697 kb on - strand at 2553.703 kb on - strand at 2553.955 kb on - strand at 2554.100 kb on + strand at 2554.142 kb on + strand, within BPHYT_RS11225 at 2554.142 kb on + strand, within BPHYT_RS11225 at 2554.311 kb on - strand, within BPHYT_RS11225 at 2554.325 kb on + strand, within BPHYT_RS11225 at 2554.401 kb on + strand, within BPHYT_RS11225 at 2554.440 kb on - strand, within BPHYT_RS11225 at 2554.679 kb on + strand, within BPHYT_RS11225 at 2554.679 kb on + strand, within BPHYT_RS11225 at 2554.679 kb on + strand, within BPHYT_RS11225 at 2554.680 kb on - strand, within BPHYT_RS11225 at 2555.003 kb on + strand at 2555.036 kb on + strand at 2555.037 kb on - strand at 2555.042 kb on - strand at 2555.046 kb on - strand at 2555.094 kb on + strand at 2555.102 kb on - strand at 2555.130 kb on - strand at 2555.178 kb on - strand at 2555.186 kb on + strand at 2555.186 kb on + strand at 2555.206 kb on - strand at 2555.362 kb on - strand, within BPHYT_RS11230 at 2555.598 kb on - strand, within BPHYT_RS11230 at 2555.820 kb on + strand at 2555.821 kb on - strand at 2555.957 kb on + strand, within BPHYT_RS11235
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 2,553,117 - BPHYT_RS11220 0.44 +0.7 2,553,178 + BPHYT_RS11220 0.50 -0.3 2,553,476 - BPHYT_RS11220 0.81 +0.4 2,553,661 - +0.2 2,553,661 - -0.7 2,553,661 - -0.0 2,553,661 - +0.5 2,553,697 - -0.5 2,553,703 - +0.3 2,553,955 - +0.2 2,554,100 + +0.4 2,554,142 + BPHYT_RS11225 0.13 +1.0 2,554,142 + BPHYT_RS11225 0.13 +1.6 2,554,311 - BPHYT_RS11225 0.30 +1.2 2,554,325 + BPHYT_RS11225 0.31 +0.7 2,554,401 + BPHYT_RS11225 0.39 +0.1 2,554,440 - BPHYT_RS11225 0.43 +1.0 2,554,679 + BPHYT_RS11225 0.68 -1.4 2,554,679 + BPHYT_RS11225 0.68 +0.0 2,554,679 + BPHYT_RS11225 0.68 +0.1 2,554,680 - BPHYT_RS11225 0.68 -0.2 2,555,003 + -0.8 2,555,036 + -0.5 2,555,037 - +0.2 2,555,042 - +0.1 2,555,046 - +1.6 2,555,094 + -0.2 2,555,102 - +0.1 2,555,130 - +0.8 2,555,178 - +1.5 2,555,186 + -0.9 2,555,186 + -0.1 2,555,206 - +0.1 2,555,362 - BPHYT_RS11230 0.33 +0.3 2,555,598 - BPHYT_RS11230 0.83 +1.1 2,555,820 + -0.5 2,555,821 - +0.1 2,555,957 + BPHYT_RS11235 0.60 -0.2
Or see this region's nucleotide sequence