Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34190 and BPHYT_RS34195 are separated by 269 nucleotides BPHYT_RS34195 and BPHYT_RS34200 are separated by 120 nucleotides BPHYT_RS34200 and BPHYT_RS34205 are separated by 58 nucleotides
BPHYT_RS34190: BPHYT_RS34190 - chemotaxis protein, at 3,273,596 to 3,275,218
_RS34190
BPHYT_RS34195: BPHYT_RS34195 - thioesterase, at 3,275,488 to 3,275,922
_RS34195
BPHYT_RS34200: BPHYT_RS34200 - ArsR family transcriptional regulator, at 3,276,043 to 3,276,345
_RS34200
BPHYT_RS34205: BPHYT_RS34205 - pyridoxal-dependent decarboxylase, at 3,276,404 to 3,277,759
_RS34205
Position (kb)
3275
3276 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3274.513 kb on + strand, within BPHYT_RS34190 at 3274.513 kb on + strand, within BPHYT_RS34190 at 3274.513 kb on + strand, within BPHYT_RS34190 at 3274.603 kb on - strand, within BPHYT_RS34190 at 3274.730 kb on - strand, within BPHYT_RS34190 at 3274.730 kb on - strand, within BPHYT_RS34190 at 3274.746 kb on + strand, within BPHYT_RS34190 at 3275.175 kb on - strand at 3275.225 kb on + strand at 3275.225 kb on + strand at 3275.253 kb on - strand at 3275.253 kb on - strand at 3275.271 kb on + strand at 3275.417 kb on - strand at 3275.417 kb on - strand at 3275.417 kb on - strand at 3275.549 kb on + strand, within BPHYT_RS34195 at 3275.550 kb on - strand, within BPHYT_RS34195 at 3275.656 kb on + strand, within BPHYT_RS34195 at 3275.657 kb on - strand, within BPHYT_RS34195 at 3275.657 kb on - strand, within BPHYT_RS34195 at 3275.657 kb on - strand, within BPHYT_RS34195 at 3275.725 kb on + strand, within BPHYT_RS34195 at 3275.726 kb on - strand, within BPHYT_RS34195 at 3275.788 kb on + strand, within BPHYT_RS34195 at 3275.827 kb on + strand, within BPHYT_RS34195 at 3275.827 kb on + strand, within BPHYT_RS34195 at 3275.828 kb on - strand, within BPHYT_RS34195 at 3276.183 kb on + strand, within BPHYT_RS34200 at 3276.286 kb on + strand, within BPHYT_RS34200 at 3276.286 kb on + strand, within BPHYT_RS34200 at 3276.287 kb on - strand, within BPHYT_RS34200 at 3276.287 kb on - strand, within BPHYT_RS34200 at 3276.289 kb on + strand, within BPHYT_RS34200 at 3276.290 kb on - strand, within BPHYT_RS34200 at 3276.290 kb on - strand, within BPHYT_RS34200 at 3276.290 kb on - strand, within BPHYT_RS34200 at 3276.290 kb on - strand, within BPHYT_RS34200 at 3276.388 kb on + strand at 3276.388 kb on + strand at 3276.388 kb on + strand at 3276.389 kb on - strand at 3276.452 kb on + strand at 3276.452 kb on + strand at 3276.453 kb on - strand at 3276.453 kb on - strand at 3276.713 kb on - strand, within BPHYT_RS34205 at 3276.713 kb on - strand, within BPHYT_RS34205
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 3,274,513 + BPHYT_RS34190 0.57 +0.3 3,274,513 + BPHYT_RS34190 0.57 +0.1 3,274,513 + BPHYT_RS34190 0.57 -0.7 3,274,603 - BPHYT_RS34190 0.62 -0.1 3,274,730 - BPHYT_RS34190 0.70 +0.4 3,274,730 - BPHYT_RS34190 0.70 +0.4 3,274,746 + BPHYT_RS34190 0.71 -0.0 3,275,175 - -0.2 3,275,225 + -0.0 3,275,225 + +0.4 3,275,253 - -1.4 3,275,253 - +0.0 3,275,271 + -2.6 3,275,417 - -1.7 3,275,417 - -1.4 3,275,417 - +1.1 3,275,549 + BPHYT_RS34195 0.14 -0.4 3,275,550 - BPHYT_RS34195 0.14 -0.7 3,275,656 + BPHYT_RS34195 0.39 +0.1 3,275,657 - BPHYT_RS34195 0.39 -0.6 3,275,657 - BPHYT_RS34195 0.39 -0.1 3,275,657 - BPHYT_RS34195 0.39 +0.5 3,275,725 + BPHYT_RS34195 0.54 +0.2 3,275,726 - BPHYT_RS34195 0.55 +1.9 3,275,788 + BPHYT_RS34195 0.69 -0.2 3,275,827 + BPHYT_RS34195 0.78 +0.3 3,275,827 + BPHYT_RS34195 0.78 +3.7 3,275,828 - BPHYT_RS34195 0.78 +0.8 3,276,183 + BPHYT_RS34200 0.46 -1.4 3,276,286 + BPHYT_RS34200 0.80 +0.1 3,276,286 + BPHYT_RS34200 0.80 -0.3 3,276,287 - BPHYT_RS34200 0.81 +0.5 3,276,287 - BPHYT_RS34200 0.81 -0.3 3,276,289 + BPHYT_RS34200 0.81 -0.0 3,276,290 - BPHYT_RS34200 0.82 -0.1 3,276,290 - BPHYT_RS34200 0.82 -0.0 3,276,290 - BPHYT_RS34200 0.82 +1.3 3,276,290 - BPHYT_RS34200 0.82 +0.3 3,276,388 + -0.3 3,276,388 + +0.2 3,276,388 + +0.3 3,276,389 - +0.7 3,276,452 + -0.0 3,276,452 + +1.2 3,276,453 - +0.7 3,276,453 - +0.5 3,276,713 - BPHYT_RS34205 0.23 -1.1 3,276,713 - BPHYT_RS34205 0.23 -0.0
Or see this region's nucleotide sequence