Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30285

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30280 and BPHYT_RS30285 are separated by 704 nucleotidesBPHYT_RS30285 and BPHYT_RS30290 are separated by 411 nucleotides BPHYT_RS30280: BPHYT_RS30280 - hypothetical protein, at 2,348,495 to 2,349,145 _RS30280 BPHYT_RS30285: BPHYT_RS30285 - succinate-semialdehyde dehydrogenase, at 2,349,850 to 2,351,322 _RS30285 BPHYT_RS30290: BPHYT_RS30290 - MFS transporter, at 2,351,734 to 2,353,092 _RS30290 Position (kb) 2349 2350 2351 2352Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2348.879 kb on + strand, within BPHYT_RS30280at 2349.113 kb on - strandat 2349.143 kb on + strandat 2349.143 kb on + strandat 2349.189 kb on + strandat 2349.189 kb on + strandat 2349.237 kb on + strandat 2349.249 kb on + strandat 2349.260 kb on - strandat 2349.516 kb on + strandat 2349.609 kb on + strandat 2350.235 kb on - strand, within BPHYT_RS30285at 2350.750 kb on + strand, within BPHYT_RS30285at 2351.245 kb on + strandat 2351.281 kb on + strandat 2351.281 kb on + strandat 2351.282 kb on - strandat 2351.282 kb on - strandat 2351.297 kb on - strandat 2351.842 kb on + strandat 2351.842 kb on + strandat 2352.088 kb on + strand, within BPHYT_RS30290

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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2,348,879 + BPHYT_RS30280 0.59 +0.5
2,349,113 - -0.2
2,349,143 + -0.1
2,349,143 + +2.0
2,349,189 + -0.1
2,349,189 + +0.1
2,349,237 + -1.3
2,349,249 + -0.4
2,349,260 - -0.3
2,349,516 + +0.2
2,349,609 + +2.3
2,350,235 - BPHYT_RS30285 0.26 -0.7
2,350,750 + BPHYT_RS30285 0.61 -3.2
2,351,245 + +0.7
2,351,281 + +0.7
2,351,281 + -1.2
2,351,282 - +0.5
2,351,282 - -0.6
2,351,297 - -1.4
2,351,842 + -0.5
2,351,842 + +0.6
2,352,088 + BPHYT_RS30290 0.26 -0.6

Or see this region's nucleotide sequence