Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22190 and BPHYT_RS22195 are separated by 22 nucleotides BPHYT_RS22195 and BPHYT_RS22200 are separated by 146 nucleotides BPHYT_RS22200 and BPHYT_RS22205 are separated by 12 nucleotides BPHYT_RS22205 and BPHYT_RS22210 overlap by 7 nucleotides
BPHYT_RS22190: BPHYT_RS22190 - nucleoid-structuring protein H-NS, at 537,221 to 538,072
_RS22190
BPHYT_RS22195: BPHYT_RS22195 - hypothetical protein, at 538,095 to 538,580
_RS22195
BPHYT_RS22200: BPHYT_RS22200 - cardiolipin synthetase, at 538,727 to 539,989
_RS22200
BPHYT_RS22205: BPHYT_RS22205 - hypothetical protein, at 540,002 to 540,958
_RS22205
BPHYT_RS22210: BPHYT_RS22210 - metal ABC transporter substrate-binding protein, at 540,952 to 541,746
_RS22210
Position (kb) 538
539
540 Strain fitness (log2 ratio) -2
-1
0
1
2 at 537.916 kb on - strand, within BPHYT_RS22190 at 538.093 kb on - strand at 538.310 kb on - strand, within BPHYT_RS22195 at 538.414 kb on + strand, within BPHYT_RS22195 at 538.833 kb on - strand at 539.347 kb on + strand, within BPHYT_RS22200 at 539.348 kb on - strand, within BPHYT_RS22200 at 539.412 kb on - strand, within BPHYT_RS22200 at 539.453 kb on - strand, within BPHYT_RS22200 at 539.889 kb on + strand at 540.025 kb on - strand at 540.237 kb on - strand, within BPHYT_RS22205 at 540.237 kb on - strand, within BPHYT_RS22205 at 540.522 kb on + strand, within BPHYT_RS22205 at 540.908 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 537,916 - BPHYT_RS22190 0.82 -0.8 538,093 - +0.7 538,310 - BPHYT_RS22195 0.44 -0.2 538,414 + BPHYT_RS22195 0.66 +2.2 538,833 - -0.4 539,347 + BPHYT_RS22200 0.49 -0.6 539,348 - BPHYT_RS22200 0.49 -0.7 539,412 - BPHYT_RS22200 0.54 -0.2 539,453 - BPHYT_RS22200 0.57 +0.8 539,889 + -2.3 540,025 - +0.2 540,237 - BPHYT_RS22205 0.25 -1.2 540,237 - BPHYT_RS22205 0.25 -0.8 540,522 + BPHYT_RS22205 0.54 +1.0 540,908 - +0.7
Or see this region's nucleotide sequence