Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21115 and BPHYT_RS21120 overlap by 8 nucleotides BPHYT_RS21120 and BPHYT_RS21125 are separated by 173 nucleotides BPHYT_RS21125 and BPHYT_RS21130 overlap by 4 nucleotides
BPHYT_RS21115: BPHYT_RS21115 - radical SAM protein, at 298,868 to 300,139
_RS21115
BPHYT_RS21120: BPHYT_RS21120 - hypothetical protein, at 300,132 to 300,959
_RS21120
BPHYT_RS21125: BPHYT_RS21125 - hypothetical protein, at 301,133 to 301,774
_RS21125
BPHYT_RS21130: BPHYT_RS21130 - F420-dependent oxidoreductase, at 301,771 to 302,550
_RS21130
Position (kb) 300
301 Strain fitness (log2 ratio) -2
-1
0
1
2 at 299.356 kb on - strand, within BPHYT_RS21115 at 299.361 kb on - strand, within BPHYT_RS21115 at 299.410 kb on + strand, within BPHYT_RS21115 at 299.555 kb on - strand, within BPHYT_RS21115 at 299.571 kb on - strand, within BPHYT_RS21115 at 299.696 kb on + strand, within BPHYT_RS21115 at 299.697 kb on - strand, within BPHYT_RS21115 at 299.749 kb on + strand, within BPHYT_RS21115 at 299.750 kb on - strand, within BPHYT_RS21115 at 299.823 kb on + strand, within BPHYT_RS21115 at 300.002 kb on + strand, within BPHYT_RS21115 at 300.068 kb on + strand at 300.313 kb on - strand, within BPHYT_RS21120 at 300.434 kb on + strand, within BPHYT_RS21120 at 300.434 kb on + strand, within BPHYT_RS21120 at 300.435 kb on - strand, within BPHYT_RS21120 at 300.507 kb on - strand, within BPHYT_RS21120 at 300.535 kb on + strand, within BPHYT_RS21120 at 300.535 kb on + strand, within BPHYT_RS21120 at 300.536 kb on - strand, within BPHYT_RS21120 at 300.918 kb on + strand at 301.132 kb on + strand at 301.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 299,356 - BPHYT_RS21115 0.38 +0.2 299,361 - BPHYT_RS21115 0.39 -0.1 299,410 + BPHYT_RS21115 0.43 +1.3 299,555 - BPHYT_RS21115 0.54 +1.2 299,571 - BPHYT_RS21115 0.55 -0.4 299,696 + BPHYT_RS21115 0.65 +0.1 299,697 - BPHYT_RS21115 0.65 -0.2 299,749 + BPHYT_RS21115 0.69 -0.9 299,750 - BPHYT_RS21115 0.69 -0.2 299,823 + BPHYT_RS21115 0.75 +0.2 300,002 + BPHYT_RS21115 0.89 -2.2 300,068 + -0.2 300,313 - BPHYT_RS21120 0.22 -1.3 300,434 + BPHYT_RS21120 0.36 -1.7 300,434 + BPHYT_RS21120 0.36 +0.3 300,435 - BPHYT_RS21120 0.37 -1.1 300,507 - BPHYT_RS21120 0.45 +2.4 300,535 + BPHYT_RS21120 0.49 +0.1 300,535 + BPHYT_RS21120 0.49 +0.2 300,536 - BPHYT_RS21120 0.49 +0.1 300,918 + +0.7 301,132 + -0.6 301,809 - +1.6
Or see this region's nucleotide sequence