Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25330 and BPHYT_RS25335 are separated by 284 nucleotides BPHYT_RS25335 and BPHYT_RS25340 are separated by 124 nucleotides BPHYT_RS25340 and BPHYT_RS25345 are separated by 56 nucleotides
BPHYT_RS25330: BPHYT_RS25330 - hypothetical protein, at 1,257,215 to 1,258,597
_RS25330
BPHYT_RS25335: BPHYT_RS25335 - hypothetical protein, at 1,258,882 to 1,259,196
_RS25335
BPHYT_RS25340: BPHYT_RS25340 - sulfatase, at 1,259,321 to 1,260,448
_RS25340
BPHYT_RS25345: BPHYT_RS25345 - arylsulfatase, at 1,260,505 to 1,262,157
_RS25345
Position (kb)
1259
1260
1261 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1258.642 kb on + strand at 1258.710 kb on - strand at 1258.715 kb on - strand at 1258.810 kb on - strand at 1258.888 kb on + strand at 1258.897 kb on - strand at 1258.934 kb on - strand, within BPHYT_RS25335 at 1259.428 kb on - strand at 1259.592 kb on + strand, within BPHYT_RS25340 at 1259.634 kb on + strand, within BPHYT_RS25340 at 1259.896 kb on - strand, within BPHYT_RS25340 at 1260.536 kb on + strand at 1260.791 kb on + strand, within BPHYT_RS25345 at 1260.864 kb on - strand, within BPHYT_RS25345 at 1261.226 kb on + strand, within BPHYT_RS25345 at 1261.227 kb on - strand, within BPHYT_RS25345 at 1261.272 kb on - strand, within BPHYT_RS25345 at 1261.415 kb on - strand, within BPHYT_RS25345
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,258,642 + +0.4 1,258,710 - +0.2 1,258,715 - +1.2 1,258,810 - -0.5 1,258,888 + +0.4 1,258,897 - -1.4 1,258,934 - BPHYT_RS25335 0.17 +0.6 1,259,428 - -1.1 1,259,592 + BPHYT_RS25340 0.24 -0.9 1,259,634 + BPHYT_RS25340 0.28 -2.5 1,259,896 - BPHYT_RS25340 0.51 -1.2 1,260,536 + -0.1 1,260,791 + BPHYT_RS25345 0.17 -1.2 1,260,864 - BPHYT_RS25345 0.22 -0.0 1,261,226 + BPHYT_RS25345 0.44 -0.5 1,261,227 - BPHYT_RS25345 0.44 -3.2 1,261,272 - BPHYT_RS25345 0.46 -0.4 1,261,415 - BPHYT_RS25345 0.55 -0.5
Or see this region's nucleotide sequence