Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22245 and BPHYT_RS22250 are separated by 74 nucleotides BPHYT_RS22250 and BPHYT_RS22255 are separated by 5 nucleotides BPHYT_RS22255 and BPHYT_RS22260 are separated by 58 nucleotides BPHYT_RS22260 and BPHYT_RS22265 are separated by 106 nucleotides
BPHYT_RS22245: BPHYT_RS22245 - transporter for D-alanine, D-lactate, and pyruvate (MctP) <small>(from data)</small>, at 548,109 to 549,590
_RS22245
BPHYT_RS22250: BPHYT_RS22250 - D-amino acid dehydrogenase, at 549,665 to 550,909
_RS22250
BPHYT_RS22255: BPHYT_RS22255 - hypothetical protein, at 550,915 to 551,259
_RS22255
BPHYT_RS22260: BPHYT_RS22260 - hypothetical protein, at 551,318 to 551,608
_RS22260
BPHYT_RS22265: BPHYT_RS22265 - hypothetical protein, at 551,715 to 552,506
_RS22265
Position (kb) 549
550
551 Strain fitness (log2 ratio) -2
-1
0
1 at 548.700 kb on + strand, within BPHYT_RS22245 at 548.700 kb on + strand, within BPHYT_RS22245 at 548.700 kb on + strand, within BPHYT_RS22245 at 548.701 kb on - strand, within BPHYT_RS22245 at 548.781 kb on + strand, within BPHYT_RS22245 at 548.782 kb on - strand, within BPHYT_RS22245 at 548.892 kb on - strand, within BPHYT_RS22245 at 549.367 kb on - strand, within BPHYT_RS22245 at 549.501 kb on + strand at 549.501 kb on + strand at 549.502 kb on - strand at 549.502 kb on - strand at 549.857 kb on - strand, within BPHYT_RS22250 at 550.087 kb on - strand, within BPHYT_RS22250 at 550.917 kb on - strand at 550.917 kb on - strand at 550.919 kb on - strand at 550.919 kb on - strand at 551.070 kb on - strand, within BPHYT_RS22255 at 551.267 kb on + strand at 551.345 kb on + strand at 551.516 kb on - strand, within BPHYT_RS22260 at 551.606 kb on + strand at 551.607 kb on - strand at 551.623 kb on + strand at 551.715 kb on - strand at 551.768 kb on - strand at 551.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 548,700 + BPHYT_RS22245 0.40 -0.7 548,700 + BPHYT_RS22245 0.40 +0.3 548,700 + BPHYT_RS22245 0.40 -0.1 548,701 - BPHYT_RS22245 0.40 +0.6 548,781 + BPHYT_RS22245 0.45 -0.3 548,782 - BPHYT_RS22245 0.45 -0.3 548,892 - BPHYT_RS22245 0.53 -0.8 549,367 - BPHYT_RS22245 0.85 -0.3 549,501 + +0.5 549,501 + -2.3 549,502 - +0.0 549,502 - -0.9 549,857 - BPHYT_RS22250 0.15 +0.8 550,087 - BPHYT_RS22250 0.34 +0.4 550,917 - -2.1 550,917 - -0.5 550,919 - -1.4 550,919 - +0.7 551,070 - BPHYT_RS22255 0.45 -0.5 551,267 + -0.2 551,345 + -0.2 551,516 - BPHYT_RS22260 0.68 +0.1 551,606 + -1.7 551,607 - -0.0 551,623 + +0.3 551,715 - +1.4 551,768 - -0.1 551,768 - -1.4
Or see this region's nucleotide sequence