Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20570 and BPHYT_RS20575 are separated by 101 nucleotides BPHYT_RS20575 and BPHYT_RS20580 are separated by 33 nucleotides BPHYT_RS20580 and BPHYT_RS20585 are separated by 229 nucleotides BPHYT_RS20585 and BPHYT_RS20590 are separated by 132 nucleotides
BPHYT_RS20570: BPHYT_RS20570 - heptosyltransferase, at 176,204 to 177,379
_RS20570
BPHYT_RS20575: BPHYT_RS20575 - ester cyclase, at 177,481 to 177,885
_RS20575
BPHYT_RS20580: BPHYT_RS20580 - NADH dehydrogenase, at 177,919 to 179,145
_RS20580
BPHYT_RS20585: BPHYT_RS20585 - MarR family transcriptional regulator, at 179,375 to 179,836
_RS20585
BPHYT_RS20590: BPHYT_RS20590 - methionine sulfoxide reductase A, at 179,969 to 180,475
_RS20590
Position (kb) 177
178
179
180 Strain fitness (log2 ratio) -2
-1
0
1 at 177.388 kb on + strand at 177.508 kb on + strand at 177.517 kb on + strand at 177.562 kb on + strand, within BPHYT_RS20575 at 177.563 kb on - strand, within BPHYT_RS20575 at 177.584 kb on - strand, within BPHYT_RS20575 at 177.584 kb on - strand, within BPHYT_RS20575 at 177.584 kb on - strand, within BPHYT_RS20575 at 177.658 kb on + strand, within BPHYT_RS20575 at 177.659 kb on - strand, within BPHYT_RS20575 at 177.695 kb on - strand at 177.697 kb on - strand, within BPHYT_RS20575 at 178.007 kb on + strand at 178.011 kb on + strand at 178.011 kb on + strand at 178.011 kb on + strand at 178.012 kb on - strand at 178.012 kb on - strand at 178.015 kb on + strand at 178.015 kb on + strand at 178.140 kb on + strand, within BPHYT_RS20580 at 178.285 kb on + strand, within BPHYT_RS20580 at 178.991 kb on + strand, within BPHYT_RS20580 at 178.992 kb on - strand, within BPHYT_RS20580 at 178.992 kb on - strand, within BPHYT_RS20580 at 179.033 kb on - strand at 179.033 kb on - strand at 179.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 177,388 + -1.1 177,508 + -0.7 177,517 + -1.2 177,562 + BPHYT_RS20575 0.20 -0.1 177,563 - BPHYT_RS20575 0.20 +0.3 177,584 - BPHYT_RS20575 0.25 +0.0 177,584 - BPHYT_RS20575 0.25 -1.1 177,584 - BPHYT_RS20575 0.25 -0.3 177,658 + BPHYT_RS20575 0.44 -0.9 177,659 - BPHYT_RS20575 0.44 -0.2 177,695 - +1.7 177,697 - BPHYT_RS20575 0.53 +1.1 178,007 + +0.2 178,011 + +0.7 178,011 + +0.4 178,011 + -2.2 178,012 - -0.6 178,012 - +0.3 178,015 + +0.1 178,015 + -1.4 178,140 + BPHYT_RS20580 0.18 -0.3 178,285 + BPHYT_RS20580 0.30 +0.1 178,991 + BPHYT_RS20580 0.87 -0.4 178,992 - BPHYT_RS20580 0.87 +0.9 178,992 - BPHYT_RS20580 0.87 +0.1 179,033 - -0.4 179,033 - -0.7 179,237 - +0.6
Or see this region's nucleotide sequence