Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20525 and BPHYT_RS20530 are separated by 128 nucleotides BPHYT_RS20530 and BPHYT_RS20535 are separated by 148 nucleotides BPHYT_RS20535 and BPHYT_RS20540 overlap by 19 nucleotides BPHYT_RS20540 and BPHYT_RS20545 are separated by 87 nucleotides
BPHYT_RS20525: BPHYT_RS20525 - esterase, at 166,985 to 168,571
_RS20525
BPHYT_RS20530: BPHYT_RS20530 - PA-phosphatase, at 168,700 to 169,302
_RS20530
BPHYT_RS20535: BPHYT_RS20535 - allantoate amidohydrolase, at 169,451 to 170,692
_RS20535
BPHYT_RS20540: BPHYT_RS20540 - N-acetyltransferase GCN5, at 170,674 to 171,555
_RS20540
BPHYT_RS20545: BPHYT_RS20545 - hypothetical protein, at 171,643 to 171,948
_RS20545
Position (kb) 169
170
171 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 168.603 kb on + strand at 168.604 kb on - strand at 168.643 kb on + strand at 169.026 kb on + strand, within BPHYT_RS20530 at 169.027 kb on - strand, within BPHYT_RS20530 at 169.191 kb on - strand, within BPHYT_RS20530 at 169.194 kb on + strand, within BPHYT_RS20530 at 169.195 kb on - strand, within BPHYT_RS20530 at 169.199 kb on + strand, within BPHYT_RS20530 at 169.200 kb on - strand, within BPHYT_RS20530 at 169.200 kb on - strand, within BPHYT_RS20530 at 169.200 kb on - strand, within BPHYT_RS20530 at 169.302 kb on + strand at 169.302 kb on + strand at 169.387 kb on - strand at 169.395 kb on + strand at 169.395 kb on + strand at 169.497 kb on + strand at 169.681 kb on + strand, within BPHYT_RS20535 at 170.285 kb on + strand, within BPHYT_RS20535 at 170.285 kb on + strand, within BPHYT_RS20535 at 170.286 kb on - strand, within BPHYT_RS20535 at 170.286 kb on - strand, within BPHYT_RS20535 at 170.286 kb on - strand, within BPHYT_RS20535 at 170.286 kb on - strand, within BPHYT_RS20535 at 170.602 kb on + strand at 170.808 kb on + strand, within BPHYT_RS20540 at 170.911 kb on + strand, within BPHYT_RS20540 at 170.912 kb on - strand, within BPHYT_RS20540 at 170.912 kb on - strand, within BPHYT_RS20540 at 170.912 kb on - strand, within BPHYT_RS20540 at 170.914 kb on - strand, within BPHYT_RS20540 at 170.977 kb on + strand, within BPHYT_RS20540 at 171.031 kb on + strand, within BPHYT_RS20540 at 171.032 kb on - strand, within BPHYT_RS20540 at 171.112 kb on + strand, within BPHYT_RS20540 at 171.488 kb on - strand at 171.509 kb on + strand at 171.644 kb on + strand at 171.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 168,603 + +0.8 168,604 - -0.5 168,643 + +2.7 169,026 + BPHYT_RS20530 0.54 -1.0 169,027 - BPHYT_RS20530 0.54 +0.3 169,191 - BPHYT_RS20530 0.81 -0.4 169,194 + BPHYT_RS20530 0.82 +0.1 169,195 - BPHYT_RS20530 0.82 -0.8 169,199 + BPHYT_RS20530 0.83 +0.5 169,200 - BPHYT_RS20530 0.83 +0.1 169,200 - BPHYT_RS20530 0.83 +0.3 169,200 - BPHYT_RS20530 0.83 +0.7 169,302 + +0.1 169,302 + +0.2 169,387 - -0.7 169,395 + -1.0 169,395 + -1.0 169,497 + +0.2 169,681 + BPHYT_RS20535 0.19 -0.1 170,285 + BPHYT_RS20535 0.67 +0.5 170,285 + BPHYT_RS20535 0.67 +3.4 170,286 - BPHYT_RS20535 0.67 -0.2 170,286 - BPHYT_RS20535 0.67 -0.3 170,286 - BPHYT_RS20535 0.67 -0.5 170,286 - BPHYT_RS20535 0.67 +0.9 170,602 + -1.9 170,808 + BPHYT_RS20540 0.15 -0.2 170,911 + BPHYT_RS20540 0.27 +0.3 170,912 - BPHYT_RS20540 0.27 -0.2 170,912 - BPHYT_RS20540 0.27 +1.1 170,912 - BPHYT_RS20540 0.27 -0.7 170,914 - BPHYT_RS20540 0.27 -0.7 170,977 + BPHYT_RS20540 0.34 -0.5 171,031 + BPHYT_RS20540 0.40 -1.3 171,032 - BPHYT_RS20540 0.41 -0.3 171,112 + BPHYT_RS20540 0.50 +0.6 171,488 - -0.1 171,509 + +1.3 171,644 + +0.8 171,645 - +0.9
Or see this region's nucleotide sequence