Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22030 and BPHYT_RS22035 overlap by 4 nucleotides BPHYT_RS22035 and BPHYT_RS22040 are separated by 1 nucleotides BPHYT_RS22040 and BPHYT_RS22045 are separated by 3 nucleotides BPHYT_RS22045 and BPHYT_RS22050 are separated by 17 nucleotides
BPHYT_RS22030: BPHYT_RS22030 - peptide ABC transporter ATP-binding protein, at 497,961 to 498,986
_RS22030
BPHYT_RS22035: BPHYT_RS22035 - peptide ABC transporter ATP-binding protein, at 498,983 to 499,981
_RS22035
BPHYT_RS22040: BPHYT_RS22040 - peptide ABC transporter permease, at 499,983 to 500,897
_RS22040
BPHYT_RS22045: BPHYT_RS22045 - peptide ABC transporter permease, at 500,901 to 501,839
_RS22045
BPHYT_RS22050: BPHYT_RS22050 - peptide ABC transporter substrate-binding protein, at 501,857 to 503,473
_RS22050
Position (kb) 499
500
501 Strain fitness (log2 ratio) -1
0
1
2
3 at 498.986 kb on + strand at 498.986 kb on + strand at 498.986 kb on + strand at 498.987 kb on - strand at 498.987 kb on - strand at 498.987 kb on - strand at 500.041 kb on + strand at 500.219 kb on - strand, within BPHYT_RS22040 at 500.446 kb on + strand, within BPHYT_RS22040 at 500.447 kb on - strand, within BPHYT_RS22040 at 500.447 kb on - strand, within BPHYT_RS22040 at 500.459 kb on - strand, within BPHYT_RS22040 at 500.933 kb on - strand at 501.652 kb on + strand, within BPHYT_RS22045 at 501.683 kb on - strand, within BPHYT_RS22045 at 501.683 kb on - strand, within BPHYT_RS22045 at 501.828 kb on - strand at 501.873 kb on + strand at 501.873 kb on + strand at 501.874 kb on - strand at 501.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 498,986 + +1.3 498,986 + +3.5 498,986 + -0.3 498,987 - -0.3 498,987 - -0.1 498,987 - -0.7 500,041 + +0.5 500,219 - BPHYT_RS22040 0.26 +1.2 500,446 + BPHYT_RS22040 0.51 +1.2 500,447 - BPHYT_RS22040 0.51 -0.6 500,447 - BPHYT_RS22040 0.51 +1.5 500,459 - BPHYT_RS22040 0.52 +0.2 500,933 - +0.2 501,652 + BPHYT_RS22045 0.80 +1.1 501,683 - BPHYT_RS22045 0.83 -1.1 501,683 - BPHYT_RS22045 0.83 +3.2 501,828 - -0.3 501,873 + +0.5 501,873 + -0.2 501,874 - +0.6 501,874 - -0.1
Or see this region's nucleotide sequence