Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS06565

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS06555 and BPHYT_RS06560 are separated by 130 nucleotidesBPHYT_RS06560 and BPHYT_RS06565 are separated by 24 nucleotidesBPHYT_RS06565 and BPHYT_RS06570 are separated by 400 nucleotides BPHYT_RS06555: BPHYT_RS06555 - ketol-acid reductoisomerase, at 1,487,893 to 1,488,909 _RS06555 BPHYT_RS06560: BPHYT_RS06560 - phosphatidylserine decarboxylase proenzyme, at 1,489,040 to 1,489,678 _RS06560 BPHYT_RS06565: BPHYT_RS06565 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,489,703 to 1,490,569 _RS06565 BPHYT_RS06570: BPHYT_RS06570 - 2-isopropylmalate synthase, at 1,490,970 to 1,492,517 _RS06570 Position (kb) 1489 1490 1491Strain fitness (log2 ratio) -1 0 1 2at 1488.749 kb on - strand, within BPHYT_RS06555at 1488.908 kb on - strandat 1488.908 kb on - strandat 1488.908 kb on - strandat 1488.947 kb on + strandat 1490.718 kb on + strandat 1490.718 kb on + strandat 1490.719 kb on - strandat 1490.989 kb on + strandat 1490.989 kb on + strandat 1491.146 kb on + strand, within BPHYT_RS06570at 1491.146 kb on + strand, within BPHYT_RS06570at 1491.507 kb on + strand, within BPHYT_RS06570at 1491.508 kb on - strand, within BPHYT_RS06570at 1491.555 kb on - strand, within BPHYT_RS06570at 1491.555 kb on - strand, within BPHYT_RS06570

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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1,488,749 - BPHYT_RS06555 0.84 -0.6
1,488,908 - +2.1
1,488,908 - -0.2
1,488,908 - -0.8
1,488,947 + +0.4
1,490,718 + -0.1
1,490,718 + -0.4
1,490,719 - -1.1
1,490,989 + +1.8
1,490,989 + -0.2
1,491,146 + BPHYT_RS06570 0.11 -0.2
1,491,146 + BPHYT_RS06570 0.11 +1.6
1,491,507 + BPHYT_RS06570 0.35 +1.0
1,491,508 - BPHYT_RS06570 0.35 -1.4
1,491,555 - BPHYT_RS06570 0.38 +0.8
1,491,555 - BPHYT_RS06570 0.38 -0.5

Or see this region's nucleotide sequence