Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06555 and BPHYT_RS06560 are separated by 130 nucleotides BPHYT_RS06560 and BPHYT_RS06565 are separated by 24 nucleotides BPHYT_RS06565 and BPHYT_RS06570 are separated by 400 nucleotides
BPHYT_RS06555: BPHYT_RS06555 - ketol-acid reductoisomerase, at 1,487,893 to 1,488,909
_RS06555
BPHYT_RS06560: BPHYT_RS06560 - phosphatidylserine decarboxylase proenzyme, at 1,489,040 to 1,489,678
_RS06560
BPHYT_RS06565: BPHYT_RS06565 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,489,703 to 1,490,569
_RS06565
BPHYT_RS06570: BPHYT_RS06570 - 2-isopropylmalate synthase, at 1,490,970 to 1,492,517
_RS06570
Position (kb)
1489
1490
1491 Strain fitness (log2 ratio)
-1
0
1
2 at 1488.749 kb on - strand, within BPHYT_RS06555 at 1488.908 kb on - strand at 1488.908 kb on - strand at 1488.908 kb on - strand at 1488.947 kb on + strand at 1490.718 kb on + strand at 1490.718 kb on + strand at 1490.719 kb on - strand at 1490.989 kb on + strand at 1490.989 kb on + strand at 1491.146 kb on + strand, within BPHYT_RS06570 at 1491.146 kb on + strand, within BPHYT_RS06570 at 1491.507 kb on + strand, within BPHYT_RS06570 at 1491.508 kb on - strand, within BPHYT_RS06570 at 1491.555 kb on - strand, within BPHYT_RS06570 at 1491.555 kb on - strand, within BPHYT_RS06570
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,488,749 - BPHYT_RS06555 0.84 -0.6 1,488,908 - +2.1 1,488,908 - -0.2 1,488,908 - -0.8 1,488,947 + +0.4 1,490,718 + -0.1 1,490,718 + -0.4 1,490,719 - -1.1 1,490,989 + +1.8 1,490,989 + -0.2 1,491,146 + BPHYT_RS06570 0.11 -0.2 1,491,146 + BPHYT_RS06570 0.11 +1.6 1,491,507 + BPHYT_RS06570 0.35 +1.0 1,491,508 - BPHYT_RS06570 0.35 -1.4 1,491,555 - BPHYT_RS06570 0.38 +0.8 1,491,555 - BPHYT_RS06570 0.38 -0.5
Or see this region's nucleotide sequence