Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35135 and BPHYT_RS35140 are separated by 215 nucleotides BPHYT_RS35140 and BPHYT_RS35145 are separated by 39 nucleotides BPHYT_RS35145 and BPHYT_RS35150 are separated by 33 nucleotides
BPHYT_RS35135: BPHYT_RS35135 - glucose-6-phosphate 1-dehydrogenase, at 3,486,380 to 3,487,987
_RS35135
BPHYT_RS35140: BPHYT_RS35140 - plasmid partitioning protein ParA, at 3,488,203 to 3,488,982
_RS35140
BPHYT_RS35145: BPHYT_RS35145 - PEBP family protein, at 3,489,022 to 3,489,591
_RS35145
BPHYT_RS35150: BPHYT_RS35150 - NADP-dependent aryl-alcohol dehydrogenase, at 3,489,625 to 3,490,620
_RS35150
Position (kb)
3488
3489 Strain fitness (log2 ratio)
-1
0
1
2 at 3487.397 kb on + strand, within BPHYT_RS35135 at 3487.443 kb on - strand, within BPHYT_RS35135 at 3487.496 kb on + strand, within BPHYT_RS35135 at 3487.496 kb on + strand, within BPHYT_RS35135 at 3487.497 kb on - strand, within BPHYT_RS35135 at 3487.767 kb on - strand, within BPHYT_RS35135 at 3487.887 kb on - strand at 3488.133 kb on + strand at 3488.707 kb on + strand, within BPHYT_RS35140 at 3488.708 kb on - strand, within BPHYT_RS35140 at 3488.788 kb on - strand, within BPHYT_RS35140 at 3488.909 kb on + strand at 3488.909 kb on + strand at 3489.259 kb on + strand, within BPHYT_RS35145 at 3489.260 kb on - strand, within BPHYT_RS35145 at 3489.322 kb on + strand, within BPHYT_RS35145 at 3489.415 kb on + strand, within BPHYT_RS35145 at 3489.418 kb on + strand, within BPHYT_RS35145 at 3489.418 kb on + strand, within BPHYT_RS35145 at 3489.457 kb on + strand, within BPHYT_RS35145 at 3489.458 kb on - strand, within BPHYT_RS35145 at 3489.651 kb on - strand at 3489.949 kb on + strand, within BPHYT_RS35150 at 3489.950 kb on - strand, within BPHYT_RS35150
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 3,487,397 + BPHYT_RS35135 0.63 +1.0 3,487,443 - BPHYT_RS35135 0.66 +0.5 3,487,496 + BPHYT_RS35135 0.69 +1.0 3,487,496 + BPHYT_RS35135 0.69 -0.8 3,487,497 - BPHYT_RS35135 0.69 -0.3 3,487,767 - BPHYT_RS35135 0.86 +0.5 3,487,887 - -0.6 3,488,133 + -0.1 3,488,707 + BPHYT_RS35140 0.65 -0.1 3,488,708 - BPHYT_RS35140 0.65 -1.0 3,488,788 - BPHYT_RS35140 0.75 -0.2 3,488,909 + -0.1 3,488,909 + -0.8 3,489,259 + BPHYT_RS35145 0.42 +0.2 3,489,260 - BPHYT_RS35145 0.42 +0.1 3,489,322 + BPHYT_RS35145 0.53 -0.9 3,489,415 + BPHYT_RS35145 0.69 +2.6 3,489,418 + BPHYT_RS35145 0.69 +1.1 3,489,418 + BPHYT_RS35145 0.69 +0.2 3,489,457 + BPHYT_RS35145 0.76 +1.3 3,489,458 - BPHYT_RS35145 0.76 -1.0 3,489,651 - -0.3 3,489,949 + BPHYT_RS35150 0.33 -0.2 3,489,950 - BPHYT_RS35150 0.33 -1.6
Or see this region's nucleotide sequence