Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31460

Experiment: R2A control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31455 and BPHYT_RS31460 are separated by 209 nucleotidesBPHYT_RS31460 and BPHYT_RS31465 overlap by 1 nucleotides BPHYT_RS31455: BPHYT_RS31455 - electron transporter RnfB, at 2,611,367 to 2,611,954 _RS31455 BPHYT_RS31460: BPHYT_RS31460 - hypothetical protein, at 2,612,164 to 2,612,457 _RS31460 BPHYT_RS31465: BPHYT_RS31465 - D-isomer specific 2-hydroxyacid dehydrogenase, at 2,612,457 to 2,613,281 _RS31465 Position (kb) 2612 2613Strain fitness (log2 ratio) -1 0 1at 2612.293 kb on + strand, within BPHYT_RS31460at 2612.294 kb on - strand, within BPHYT_RS31460at 2612.703 kb on + strand, within BPHYT_RS31465at 2612.908 kb on - strand, within BPHYT_RS31465at 2613.365 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A control
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2,612,293 + BPHYT_RS31460 0.44 +0.3
2,612,294 - BPHYT_RS31460 0.44 +0.8
2,612,703 + BPHYT_RS31465 0.30 -0.6
2,612,908 - BPHYT_RS31465 0.55 -1.0
2,613,365 + -0.4

Or see this region's nucleotide sequence