Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23465 and BPHYT_RS23470 are separated by 89 nucleotides BPHYT_RS23470 and BPHYT_RS23475 are separated by 72 nucleotides BPHYT_RS23475 and BPHYT_RS23480 are separated by 339 nucleotides BPHYT_RS23480 and BPHYT_RS23485 are separated by 27 nucleotides
BPHYT_RS23465: BPHYT_RS23465 - aliphatic nitrilase, at 832,323 to 833,309
_RS23465
BPHYT_RS23470: BPHYT_RS23470 - carbonic anhydrase, at 833,399 to 833,953
_RS23470
BPHYT_RS23475: BPHYT_RS23475 - luciferase, at 834,026 to 835,033
_RS23475
BPHYT_RS23480: BPHYT_RS23480 - hypothetical protein, at 835,373 to 835,894
_RS23480
BPHYT_RS23485: BPHYT_RS23485 - glyoxalase, at 835,922 to 836,344
_RS23485
Position (kb) 834
835
836 Strain fitness (log2 ratio) -2
-1
0
1
2 at 833.085 kb on + strand, within BPHYT_RS23465 at 833.085 kb on + strand, within BPHYT_RS23465 at 833.447 kb on - strand at 833.488 kb on + strand, within BPHYT_RS23470 at 833.714 kb on - strand, within BPHYT_RS23470 at 834.084 kb on + strand at 834.177 kb on - strand, within BPHYT_RS23475 at 834.202 kb on + strand, within BPHYT_RS23475 at 835.028 kb on - strand at 835.172 kb on - strand at 835.280 kb on + strand at 835.326 kb on + strand at 835.327 kb on - strand at 835.535 kb on + strand, within BPHYT_RS23480 at 835.536 kb on - strand, within BPHYT_RS23480 at 835.917 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 833,085 + BPHYT_RS23465 0.77 +2.0 833,085 + BPHYT_RS23465 0.77 -0.2 833,447 - -0.3 833,488 + BPHYT_RS23470 0.16 -0.3 833,714 - BPHYT_RS23470 0.57 -0.0 834,084 + -0.6 834,177 - BPHYT_RS23475 0.15 +0.2 834,202 + BPHYT_RS23475 0.17 -2.0 835,028 - -0.3 835,172 - +1.0 835,280 + -0.0 835,326 + +0.4 835,327 - -0.2 835,535 + BPHYT_RS23480 0.31 +0.5 835,536 - BPHYT_RS23480 0.31 -0.4 835,917 - +0.1
Or see this region's nucleotide sequence