Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20925 and BPHYT_RS20930 are separated by 584 nucleotides BPHYT_RS20930 and BPHYT_RS20935 are separated by 37 nucleotides BPHYT_RS20935 and BPHYT_RS20940 are separated by 146 nucleotides
BPHYT_RS20925: BPHYT_RS20925 - dimethylallyltransferase, at 256,392 to 257,069
_RS20925
BPHYT_RS20930: BPHYT_RS20930 - hypothetical protein, at 257,654 to 257,941
_RS20930
BPHYT_RS20935: BPHYT_RS20935 - dehydrogenase, at 257,979 to 258,413
_RS20935
BPHYT_RS20940: BPHYT_RS20940 - citrate synthase, at 258,560 to 259,804
_RS20940
Position (kb) 257
258
259 Strain fitness (log2 ratio) -1
0
1 at 257.431 kb on + strand at 257.432 kb on - strand at 257.541 kb on + strand at 257.541 kb on + strand at 257.558 kb on + strand at 257.596 kb on + strand at 257.596 kb on + strand at 257.596 kb on + strand at 257.599 kb on + strand at 257.648 kb on + strand at 257.649 kb on - strand at 257.649 kb on - strand at 257.649 kb on - strand at 257.664 kb on - strand at 257.715 kb on - strand, within BPHYT_RS20930 at 257.715 kb on - strand, within BPHYT_RS20930 at 257.981 kb on - strand at 257.988 kb on - strand at 257.988 kb on - strand at 258.037 kb on - strand, within BPHYT_RS20935 at 258.047 kb on + strand, within BPHYT_RS20935 at 258.048 kb on - strand, within BPHYT_RS20935 at 258.275 kb on + strand, within BPHYT_RS20935
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 257,431 + +0.5 257,432 - -0.2 257,541 + +0.6 257,541 + +0.5 257,558 + -0.3 257,596 + +0.3 257,596 + +0.5 257,596 + +0.4 257,599 + +0.2 257,648 + -1.3 257,649 - -0.3 257,649 - -0.2 257,649 - +0.2 257,664 - -0.6 257,715 - BPHYT_RS20930 0.21 +0.0 257,715 - BPHYT_RS20930 0.21 -0.6 257,981 - +0.3 257,988 - -0.4 257,988 - -0.4 258,037 - BPHYT_RS20935 0.13 +0.5 258,047 + BPHYT_RS20935 0.16 +0.1 258,048 - BPHYT_RS20935 0.16 +1.1 258,275 + BPHYT_RS20935 0.68 +0.5
Or see this region's nucleotide sequence