Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11000

Experiment: R2A control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10995 and BPHYT_RS11000 are separated by 280 nucleotidesBPHYT_RS11000 and BPHYT_RS11005 are separated by 16 nucleotidesBPHYT_RS11005 and BPHYT_RS11010 overlap by 11 nucleotides BPHYT_RS10995: BPHYT_RS10995 - GntR family transcriptional regulator, at 2,499,275 to 2,499,994 _RS10995 BPHYT_RS11000: BPHYT_RS11000 - glutathione S-transferase, at 2,500,275 to 2,500,916 _RS11000 BPHYT_RS11005: BPHYT_RS11005 - chromate transporter, at 2,500,933 to 2,501,469 _RS11005 BPHYT_RS11010: BPHYT_RS11010 - chromate transporter, at 2,501,459 to 2,502,022 _RS11010 Position (kb) 2500 2501Strain fitness (log2 ratio) -1 0 1at 2499.570 kb on + strand, within BPHYT_RS10995at 2499.571 kb on - strand, within BPHYT_RS10995at 2500.456 kb on + strand, within BPHYT_RS11000at 2500.457 kb on - strand, within BPHYT_RS11000at 2500.679 kb on - strand, within BPHYT_RS11000at 2500.930 kb on + strandat 2501.098 kb on + strand, within BPHYT_RS11005at 2501.454 kb on - strandat 2501.493 kb on + strandat 2501.493 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A control
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2,499,570 + BPHYT_RS10995 0.41 +1.0
2,499,571 - BPHYT_RS10995 0.41 +0.3
2,500,456 + BPHYT_RS11000 0.28 -0.4
2,500,457 - BPHYT_RS11000 0.28 -0.8
2,500,679 - BPHYT_RS11000 0.63 -0.8
2,500,930 + +0.1
2,501,098 + BPHYT_RS11005 0.31 -0.7
2,501,454 - +0.3
2,501,493 + -0.2
2,501,493 + -0.4

Or see this region's nucleotide sequence