Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31625

Experiment: R2A with Vogels_fungal_media 0.8X

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31620 and BPHYT_RS31625 are separated by 17 nucleotidesBPHYT_RS31625 and BPHYT_RS31630 overlap by 4 nucleotidesBPHYT_RS31630 and BPHYT_RS31635 overlap by 8 nucleotides BPHYT_RS31620: BPHYT_RS31620 - mandelate racemase, at 2,647,458 to 2,648,579 _RS31620 BPHYT_RS31625: BPHYT_RS31625 - membrane protein, at 2,648,597 to 2,649,601 _RS31625 BPHYT_RS31630: BPHYT_RS31630 - hypothetical protein, at 2,649,598 to 2,650,260 _RS31630 BPHYT_RS31635: BPHYT_RS31635 - choline dehydrogenase, at 2,650,253 to 2,651,896 _RS31635 Position (kb) 2648 2649 2650Strain fitness (log2 ratio) -2 -1 0 1at 2647.635 kb on + strand, within BPHYT_RS31620at 2647.637 kb on + strand, within BPHYT_RS31620at 2647.881 kb on + strand, within BPHYT_RS31620at 2648.093 kb on - strand, within BPHYT_RS31620at 2648.582 kb on - strandat 2648.626 kb on - strandat 2648.909 kb on + strand, within BPHYT_RS31625at 2649.005 kb on + strand, within BPHYT_RS31625at 2649.317 kb on + strand, within BPHYT_RS31625at 2650.100 kb on - strand, within BPHYT_RS31630at 2650.100 kb on - strand, within BPHYT_RS31630at 2650.167 kb on - strand, within BPHYT_RS31630at 2650.309 kb on - strandat 2650.309 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X
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2,647,635 + BPHYT_RS31620 0.16 +0.4
2,647,637 + BPHYT_RS31620 0.16 -0.7
2,647,881 + BPHYT_RS31620 0.38 -1.1
2,648,093 - BPHYT_RS31620 0.57 +0.7
2,648,582 - -0.0
2,648,626 - +1.6
2,648,909 + BPHYT_RS31625 0.31 +0.7
2,649,005 + BPHYT_RS31625 0.41 +1.5
2,649,317 + BPHYT_RS31625 0.72 -0.0
2,650,100 - BPHYT_RS31630 0.76 -0.0
2,650,100 - BPHYT_RS31630 0.76 -0.2
2,650,167 - BPHYT_RS31630 0.86 +0.0
2,650,309 - -2.0
2,650,309 - -0.4

Or see this region's nucleotide sequence