Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28150 and BPHYT_RS28155 are separated by 8 nucleotides BPHYT_RS28155 and BPHYT_RS28160 are separated by 75 nucleotides BPHYT_RS28160 and BPHYT_RS28165 are separated by 178 nucleotides BPHYT_RS28165 and BPHYT_RS28170 are separated by 10 nucleotides
BPHYT_RS28150: BPHYT_RS28150 - methylmalonate-semialdehyde dehydrogenase, at 1,873,333 to 1,874,850
_RS28150
BPHYT_RS28155: BPHYT_RS28155 - hypothetical protein, at 1,874,859 to 1,875,125
_RS28155
BPHYT_RS28160: BPHYT_RS28160 - MFS transporter, at 1,875,201 to 1,876,445
_RS28160
BPHYT_RS28165: BPHYT_RS28165 - histidine kinase, at 1,876,624 to 1,877,199
_RS28165
BPHYT_RS28170: BPHYT_RS28170 - nitrate ABC transporter substrate-binding protein, at 1,877,210 to 1,878,361
_RS28170
Position (kb) 1875
1876
1877 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1874.565 kb on + strand, within BPHYT_RS28150 at 1874.758 kb on + strand at 1874.759 kb on - strand at 1874.955 kb on - strand, within BPHYT_RS28155 at 1874.967 kb on - strand, within BPHYT_RS28155 at 1875.097 kb on + strand, within BPHYT_RS28155 at 1875.933 kb on - strand, within BPHYT_RS28160 at 1875.964 kb on - strand, within BPHYT_RS28160 at 1876.532 kb on - strand at 1877.107 kb on + strand, within BPHYT_RS28165
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 1,874,565 + BPHYT_RS28150 0.81 +0.6 1,874,758 + -0.3 1,874,759 - -2.8 1,874,955 - BPHYT_RS28155 0.36 +0.0 1,874,967 - BPHYT_RS28155 0.40 -0.3 1,875,097 + BPHYT_RS28155 0.89 +0.2 1,875,933 - BPHYT_RS28160 0.59 -0.0 1,875,964 - BPHYT_RS28160 0.61 +1.2 1,876,532 - -1.5 1,877,107 + BPHYT_RS28165 0.84 +0.3
Or see this region's nucleotide sequence