Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27355 and BPHYT_RS27360 overlap by 4 nucleotides BPHYT_RS27360 and BPHYT_RS27365 are separated by 1 nucleotides BPHYT_RS27365 and BPHYT_RS27370 overlap by 1 nucleotides BPHYT_RS27370 and BPHYT_RS27375 overlap by 1 nucleotides BPHYT_RS27375 and BPHYT_RS27380 are separated by 8 nucleotides
BPHYT_RS27355: BPHYT_RS27355 - 2-dehydropantoate 2-reductase, at 1,712,694 to 1,713,665
_RS27355
BPHYT_RS27360: BPHYT_RS27360 - glyoxalase, at 1,713,662 to 1,714,054
_RS27360
BPHYT_RS27365: BPHYT_RS27365 - acyl dehydratase, at 1,714,056 to 1,714,514
_RS27365
BPHYT_RS27370: BPHYT_RS27370 - CoA transferase, at 1,714,514 to 1,715,734
_RS27370
BPHYT_RS27375: BPHYT_RS27375 - shikimate 5-dehydrogenase, at 1,715,734 to 1,716,567
_RS27375
BPHYT_RS27380: BPHYT_RS27380 - 3-dehydroquinate dehydratase, at 1,716,576 to 1,716,683
_RS27380
Position (kb) 1714
1715
1716 Strain fitness (log2 ratio) -1
0
1 at 1713.680 kb on + strand at 1714.120 kb on - strand, within BPHYT_RS27365 at 1715.028 kb on - strand, within BPHYT_RS27370 at 1715.118 kb on - strand, within BPHYT_RS27370 at 1715.145 kb on - strand, within BPHYT_RS27370 at 1715.220 kb on - strand, within BPHYT_RS27370 at 1715.856 kb on - strand, within BPHYT_RS27375 at 1716.265 kb on + strand, within BPHYT_RS27375
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 1,713,680 + +0.0 1,714,120 - BPHYT_RS27365 0.14 -1.5 1,715,028 - BPHYT_RS27370 0.42 -0.9 1,715,118 - BPHYT_RS27370 0.49 -1.3 1,715,145 - BPHYT_RS27370 0.52 +1.5 1,715,220 - BPHYT_RS27370 0.58 -0.4 1,715,856 - BPHYT_RS27375 0.15 -0.0 1,716,265 + BPHYT_RS27375 0.64 -0.0
Or see this region's nucleotide sequence