Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07120 and BPHYT_RS07125 are separated by 76 nucleotides BPHYT_RS07125 and BPHYT_RS07130 are separated by 12 nucleotides BPHYT_RS07130 and BPHYT_RS07135 are separated by 24 nucleotides BPHYT_RS07135 and BPHYT_RS07140 are separated by 9 nucleotides
BPHYT_RS07120: BPHYT_RS07120 - biopolymer transporter TonB, at 1,615,573 to 1,616,280
_RS07120
BPHYT_RS07125: BPHYT_RS07125 - biopolymer transporter ExbB, at 1,616,357 to 1,617,262
_RS07125
BPHYT_RS07130: BPHYT_RS07130 - biopolymer transporter ExbD, at 1,617,275 to 1,617,697
_RS07130
BPHYT_RS07135: BPHYT_RS07135 - biopolymer transporter ExbD, at 1,617,722 to 1,618,150
_RS07135
BPHYT_RS07140: BPHYT_RS07140 - hypothetical protein, at 1,618,160 to 1,619,344
_RS07140
Position (kb) 1617
1618 Strain fitness (log2 ratio) -1
0
1 at 1616.338 kb on + strand at 1616.576 kb on + strand, within BPHYT_RS07125 at 1617.460 kb on + strand, within BPHYT_RS07130 at 1618.305 kb on - strand, within BPHYT_RS07140 at 1618.619 kb on + strand, within BPHYT_RS07140
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 1,616,338 + -0.1 1,616,576 + BPHYT_RS07125 0.24 -0.9 1,617,460 + BPHYT_RS07130 0.44 -1.5 1,618,305 - BPHYT_RS07140 0.12 +0.7 1,618,619 + BPHYT_RS07140 0.39 +0.0
Or see this region's nucleotide sequence