Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00850 and BPHYT_RS00855 are separated by 661 nucleotides BPHYT_RS00855 and BPHYT_RS00860 are separated by 833 nucleotides
BPHYT_RS00850: BPHYT_RS00850 - hypothetical protein, at 192,467 to 192,898
_RS00850
BPHYT_RS00855: BPHYT_RS00855 - hypothetical protein, at 193,560 to 193,820
_RS00855
BPHYT_RS00860: BPHYT_RS00860 - 3-ketoacyl-ACP reductase, at 194,654 to 195,394
_RS00860
Position (kb) 193
194 Strain fitness (log2 ratio) -1
0
1
2 at 192.711 kb on + strand, within BPHYT_RS00850 at 192.711 kb on + strand, within BPHYT_RS00850 at 192.711 kb on + strand, within BPHYT_RS00850 at 192.711 kb on + strand, within BPHYT_RS00850 at 192.711 kb on + strand, within BPHYT_RS00850 at 192.712 kb on - strand, within BPHYT_RS00850 at 192.712 kb on - strand, within BPHYT_RS00850 at 192.712 kb on - strand, within BPHYT_RS00850 at 192.712 kb on - strand, within BPHYT_RS00850 at 192.863 kb on - strand at 192.987 kb on + strand at 192.988 kb on - strand at 192.988 kb on - strand at 192.988 kb on - strand at 192.988 kb on - strand at 192.989 kb on + strand at 193.049 kb on + strand at 193.049 kb on + strand at 193.075 kb on + strand at 193.122 kb on - strand at 193.122 kb on - strand at 193.125 kb on + strand at 193.126 kb on - strand at 193.190 kb on + strand at 193.190 kb on + strand at 193.191 kb on - strand at 193.475 kb on + strand at 193.475 kb on + strand at 193.476 kb on - strand at 193.476 kb on - strand at 193.673 kb on + strand, within BPHYT_RS00855 at 194.016 kb on - strand at 194.103 kb on - strand at 194.127 kb on + strand at 194.206 kb on - strand at 194.348 kb on - strand at 194.399 kb on + strand at 194.400 kb on - strand at 194.662 kb on + strand at 194.662 kb on + strand at 194.797 kb on + strand, within BPHYT_RS00860 at 194.797 kb on + strand, within BPHYT_RS00860 at 194.801 kb on + strand, within BPHYT_RS00860 at 194.802 kb on - strand, within BPHYT_RS00860 at 194.802 kb on - strand, within BPHYT_RS00860
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 192,711 + BPHYT_RS00850 0.56 +0.2 192,711 + BPHYT_RS00850 0.56 -0.2 192,711 + BPHYT_RS00850 0.56 -0.2 192,711 + BPHYT_RS00850 0.56 +0.3 192,711 + BPHYT_RS00850 0.56 +0.8 192,712 - BPHYT_RS00850 0.57 +0.7 192,712 - BPHYT_RS00850 0.57 -0.7 192,712 - BPHYT_RS00850 0.57 +0.6 192,712 - BPHYT_RS00850 0.57 -1.0 192,863 - -1.5 192,987 + +0.0 192,988 - -0.2 192,988 - -0.1 192,988 - +2.6 192,988 - -0.4 192,989 + -0.2 193,049 + +0.3 193,049 + -0.2 193,075 + -0.7 193,122 - +0.0 193,122 - -0.3 193,125 + +1.1 193,126 - -0.2 193,190 + +1.0 193,190 + -0.2 193,191 - -0.3 193,475 + +0.1 193,475 + +0.0 193,476 - +0.4 193,476 - -1.3 193,673 + BPHYT_RS00855 0.43 +0.6 194,016 - -0.0 194,103 - -0.7 194,127 + +0.7 194,206 - -0.7 194,348 - -0.2 194,399 + +0.7 194,400 - -1.6 194,662 + -0.4 194,662 + +0.9 194,797 + BPHYT_RS00860 0.19 -0.1 194,797 + BPHYT_RS00860 0.19 +0.8 194,801 + BPHYT_RS00860 0.20 -1.5 194,802 - BPHYT_RS00860 0.20 +0.2 194,802 - BPHYT_RS00860 0.20 +0.4
Or see this region's nucleotide sequence