Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24580 and BPHYT_RS24585 are separated by 222 nucleotides BPHYT_RS24585 and BPHYT_RS24590 are separated by 116 nucleotides BPHYT_RS24590 and BPHYT_RS24595 are separated by 483 nucleotides
BPHYT_RS24580: BPHYT_RS24580 - hypothetical protein, at 1,084,272 to 1,084,601
_RS24580
BPHYT_RS24585: BPHYT_RS24585 - NUDIX hydrolase, at 1,084,824 to 1,085,411
_RS24585
BPHYT_RS24590: BPHYT_RS24590 - DeoR faimly transcriptional regulator, at 1,085,528 to 1,086,289
_RS24590
BPHYT_RS24595: BPHYT_RS24595 - aldehyde oxidase, at 1,086,773 to 1,089,601
_RS24595
Position (kb)
1085
1086
1087 Strain fitness (log2 ratio)
-1
0
1 at 1084.737 kb on - strand at 1084.742 kb on + strand at 1084.999 kb on + strand, within BPHYT_RS24585 at 1086.098 kb on + strand, within BPHYT_RS24590 at 1086.236 kb on - strand at 1086.333 kb on + strand at 1086.333 kb on + strand at 1086.675 kb on + strand at 1087.070 kb on + strand, within BPHYT_RS24595 at 1087.070 kb on + strand, within BPHYT_RS24595 at 1087.070 kb on + strand, within BPHYT_RS24595 at 1087.071 kb on - strand, within BPHYT_RS24595 at 1087.172 kb on + strand, within BPHYT_RS24595 at 1087.214 kb on + strand, within BPHYT_RS24595
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 1,084,737 - -0.9 1,084,742 + +0.3 1,084,999 + BPHYT_RS24585 0.30 -0.6 1,086,098 + BPHYT_RS24590 0.75 -1.0 1,086,236 - -0.2 1,086,333 + -0.0 1,086,333 + +0.2 1,086,675 + -0.6 1,087,070 + BPHYT_RS24595 0.10 -0.5 1,087,070 + BPHYT_RS24595 0.10 -0.8 1,087,070 + BPHYT_RS24595 0.10 +1.3 1,087,071 - BPHYT_RS24595 0.11 -1.2 1,087,172 + BPHYT_RS24595 0.14 -1.2 1,087,214 + BPHYT_RS24595 0.16 -0.0
Or see this region's nucleotide sequence