Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23045 and BPHYT_RS23050 are separated by 131 nucleotides BPHYT_RS23050 and BPHYT_RS23055 are separated by 272 nucleotides BPHYT_RS23055 and BPHYT_RS23060 are separated by 201 nucleotides
BPHYT_RS23045: BPHYT_RS23045 - AsnC family transcriptional regulator, at 732,189 to 732,854
_RS23045
BPHYT_RS23050: BPHYT_RS23050 - hypothetical protein, at 732,986 to 733,306
_RS23050
BPHYT_RS23055: BPHYT_RS23055 - LysR family transcriptional regulator, at 733,579 to 734,481
_RS23055
BPHYT_RS23060: BPHYT_RS23060 - 3-isopropylmalate dehydratase large subunit, at 734,683 to 736,650
_RS23060
Position (kb) 733
734
735 Strain fitness (log2 ratio) -1
0
1 at 733.046 kb on + strand, within BPHYT_RS23050 at 733.433 kb on + strand at 733.434 kb on - strand at 733.549 kb on + strand at 734.536 kb on + strand at 734.660 kb on + strand at 734.660 kb on + strand at 734.682 kb on + strand at 734.683 kb on - strand at 734.683 kb on - strand at 734.683 kb on - strand at 734.719 kb on + strand at 734.720 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 733,046 + BPHYT_RS23050 0.19 -0.5 733,433 + +0.0 733,434 - +0.3 733,549 + +0.5 734,536 + +0.3 734,660 + -0.0 734,660 + -0.4 734,682 + +0.8 734,683 - -0.9 734,683 - -0.6 734,683 - -0.6 734,719 + +0.2 734,720 - +0.2
Or see this region's nucleotide sequence