Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21410 and BPHYT_RS21415 are separated by 386 nucleotides BPHYT_RS21415 and BPHYT_RS21420 are separated by 13 nucleotides BPHYT_RS21420 and BPHYT_RS21425 are separated by 92 nucleotides
BPHYT_RS21410: BPHYT_RS21410 - LysR family transcriptional regulator, at 362,288 to 363,217
_RS21410
BPHYT_RS21415: BPHYT_RS21415 - 3-oxoadipate CoA-transferase subunit A, at 363,604 to 364,311
_RS21415
BPHYT_RS21420: BPHYT_RS21420 - 3-oxoadipate CoA-transferase subunit B, at 364,325 to 364,984
_RS21420
BPHYT_RS21425: BPHYT_RS21425 - 3-carboxy-cis,cis-muconate cycloisomerase, at 365,077 to 366,456
_RS21425
Position (kb) 363
364
365 Strain fitness (log2 ratio) -2
-1
0
1
2 at 362.643 kb on + strand, within BPHYT_RS21410 at 362.643 kb on + strand, within BPHYT_RS21410 at 362.643 kb on + strand, within BPHYT_RS21410 at 362.643 kb on + strand, within BPHYT_RS21410 at 362.644 kb on - strand, within BPHYT_RS21410 at 362.644 kb on - strand, within BPHYT_RS21410 at 362.644 kb on - strand, within BPHYT_RS21410 at 362.737 kb on + strand, within BPHYT_RS21410 at 362.737 kb on + strand, within BPHYT_RS21410 at 362.738 kb on - strand, within BPHYT_RS21410 at 362.926 kb on + strand, within BPHYT_RS21410 at 362.927 kb on - strand, within BPHYT_RS21410 at 362.927 kb on - strand, within BPHYT_RS21410 at 362.957 kb on + strand, within BPHYT_RS21410 at 363.262 kb on - strand at 363.362 kb on + strand at 363.362 kb on + strand at 363.363 kb on - strand at 363.363 kb on - strand at 363.369 kb on - strand at 363.412 kb on + strand at 363.452 kb on - strand at 363.856 kb on + strand, within BPHYT_RS21415 at 363.857 kb on - strand, within BPHYT_RS21415 at 363.874 kb on + strand, within BPHYT_RS21415 at 363.874 kb on + strand, within BPHYT_RS21415 at 364.112 kb on - strand, within BPHYT_RS21415 at 364.388 kb on + strand at 364.414 kb on + strand, within BPHYT_RS21420 at 364.571 kb on + strand, within BPHYT_RS21420 at 364.748 kb on + strand, within BPHYT_RS21420 at 364.844 kb on + strand, within BPHYT_RS21420 at 364.845 kb on - strand, within BPHYT_RS21420 at 365.038 kb on + strand at 365.038 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 362,643 + BPHYT_RS21410 0.38 -2.6 362,643 + BPHYT_RS21410 0.38 +1.9 362,643 + BPHYT_RS21410 0.38 -0.8 362,643 + BPHYT_RS21410 0.38 -0.1 362,644 - BPHYT_RS21410 0.38 -0.4 362,644 - BPHYT_RS21410 0.38 -0.3 362,644 - BPHYT_RS21410 0.38 +0.1 362,737 + BPHYT_RS21410 0.48 -0.2 362,737 + BPHYT_RS21410 0.48 +0.2 362,738 - BPHYT_RS21410 0.48 -1.1 362,926 + BPHYT_RS21410 0.69 +0.6 362,927 - BPHYT_RS21410 0.69 -0.1 362,927 - BPHYT_RS21410 0.69 +0.3 362,957 + BPHYT_RS21410 0.72 +1.0 363,262 - +0.1 363,362 + -0.6 363,362 + -0.4 363,363 - -0.1 363,363 - -0.4 363,369 - -0.1 363,412 + -0.0 363,452 - +0.2 363,856 + BPHYT_RS21415 0.36 +0.2 363,857 - BPHYT_RS21415 0.36 -0.6 363,874 + BPHYT_RS21415 0.38 -0.9 363,874 + BPHYT_RS21415 0.38 -1.0 364,112 - BPHYT_RS21415 0.72 -1.4 364,388 + +1.1 364,414 + BPHYT_RS21420 0.13 +0.9 364,571 + BPHYT_RS21420 0.37 +0.6 364,748 + BPHYT_RS21420 0.64 +0.8 364,844 + BPHYT_RS21420 0.79 -0.3 364,845 - BPHYT_RS21420 0.79 -0.2 365,038 + -2.1 365,038 + -0.6
Or see this region's nucleotide sequence