Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14580 and BPHYT_RS14585 are separated by 243 nucleotides BPHYT_RS14585 and BPHYT_RS14590 are separated by 102 nucleotides BPHYT_RS14590 and BPHYT_RS14595 are separated by 314 nucleotides
BPHYT_RS14580: BPHYT_RS14580 - ATP-dependent DNA helicase, at 3,320,244 to 3,322,406
_RS14580
BPHYT_RS14585: BPHYT_RS14585 - hypothetical protein, at 3,322,650 to 3,322,913
_RS14585
BPHYT_RS14590: BPHYT_RS14590 - hypothetical protein, at 3,323,016 to 3,323,462
_RS14590
BPHYT_RS14595: BPHYT_RS14595 - RNA-binding protein, at 3,323,777 to 3,323,995
_RS14595
Position (kb)
3322
3323 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3321.751 kb on + strand, within BPHYT_RS14580 at 3321.752 kb on - strand, within BPHYT_RS14580 at 3322.286 kb on - strand at 3322.447 kb on + strand at 3322.452 kb on + strand at 3322.454 kb on + strand at 3322.460 kb on + strand at 3322.651 kb on + strand at 3322.652 kb on - strand at 3322.805 kb on + strand, within BPHYT_RS14585 at 3322.830 kb on - strand, within BPHYT_RS14585 at 3322.918 kb on + strand at 3323.017 kb on + strand at 3323.017 kb on + strand at 3323.017 kb on + strand at 3323.143 kb on + strand, within BPHYT_RS14590 at 3323.144 kb on - strand, within BPHYT_RS14590 at 3323.144 kb on - strand, within BPHYT_RS14590 at 3323.144 kb on - strand, within BPHYT_RS14590 at 3323.448 kb on - strand at 3323.448 kb on - strand at 3323.527 kb on - strand at 3323.608 kb on - strand at 3323.669 kb on + strand at 3323.669 kb on + strand at 3323.669 kb on + strand at 3323.670 kb on - strand at 3323.706 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 3,321,751 + BPHYT_RS14580 0.70 -0.8 3,321,752 - BPHYT_RS14580 0.70 -0.0 3,322,286 - -0.0 3,322,447 + +0.2 3,322,452 + +0.3 3,322,454 + +0.4 3,322,460 + +0.9 3,322,651 + -0.6 3,322,652 - +0.2 3,322,805 + BPHYT_RS14585 0.59 -1.6 3,322,830 - BPHYT_RS14585 0.68 -0.6 3,322,918 + +0.3 3,323,017 + -0.3 3,323,017 + +0.4 3,323,017 + -0.3 3,323,143 + BPHYT_RS14590 0.28 -0.1 3,323,144 - BPHYT_RS14590 0.29 -0.2 3,323,144 - BPHYT_RS14590 0.29 -0.2 3,323,144 - BPHYT_RS14590 0.29 +0.2 3,323,448 - +1.3 3,323,448 - +2.8 3,323,527 - -0.4 3,323,608 - +0.1 3,323,669 + +0.3 3,323,669 + +0.4 3,323,669 + -0.8 3,323,670 - +0.1 3,323,706 - +0.6
Or see this region's nucleotide sequence