Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36080 and BPHYT_RS36085 overlap by 4 nucleotides BPHYT_RS36085 and BPHYT_RS36090 are separated by 428 nucleotides BPHYT_RS36090 and BPHYT_RS36095 are separated by 19 nucleotides BPHYT_RS36095 and BPHYT_RS36100 are separated by 128 nucleotides BPHYT_RS36100 and BPHYT_RS36105 are separated by 54 nucleotides
BPHYT_RS36080: BPHYT_RS36080 - hypothetical protein, at 40,568 to 40,915
_RS36080
BPHYT_RS36085: BPHYT_RS36085 - hypothetical protein, at 40,912 to 41,403
_RS36085
BPHYT_RS36090: BPHYT_RS36090 - hypothetical protein, at 41,832 to 42,038
_RS36090
BPHYT_RS36095: BPHYT_RS36095 - hypothetical protein, at 42,058 to 42,282
_RS36095
BPHYT_RS36100: BPHYT_RS36100 - hypothetical protein, at 42,411 to 42,680
_RS36100
BPHYT_RS36105: BPHYT_RS36105 - hypothetical protein, at 42,735 to 43,067
_RS36105
Position (kb) 41
42
43 Strain fitness (log2 ratio) -1
0
1
2 at 40.887 kb on + strand at 41.292 kb on - strand, within BPHYT_RS36085 at 41.814 kb on - strand at 41.861 kb on + strand, within BPHYT_RS36090 at 41.861 kb on + strand, within BPHYT_RS36090 at 41.862 kb on - strand, within BPHYT_RS36090 at 42.371 kb on - strand at 42.504 kb on + strand, within BPHYT_RS36100 at 42.658 kb on - strand at 42.866 kb on - strand, within BPHYT_RS36105
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 40,887 + -0.1 41,292 - BPHYT_RS36085 0.77 +0.8 41,814 - +1.5 41,861 + BPHYT_RS36090 0.14 +2.1 41,861 + BPHYT_RS36090 0.14 +0.1 41,862 - BPHYT_RS36090 0.14 +0.6 42,371 - -0.5 42,504 + BPHYT_RS36100 0.34 -1.7 42,658 - +0.9 42,866 - BPHYT_RS36105 0.39 -1.6
Or see this region's nucleotide sequence