Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24490 and BPHYT_RS24495 are separated by 193 nucleotides BPHYT_RS24495 and BPHYT_RS24500 overlap by 4 nucleotides BPHYT_RS24500 and BPHYT_RS24505 overlap by 4 nucleotides BPHYT_RS24505 and BPHYT_RS24510 are separated by 54 nucleotides
BPHYT_RS24490: BPHYT_RS24490 - hypothetical protein, at 1,063,250 to 1,063,882
_RS24490
BPHYT_RS24495: BPHYT_RS24495 - transcriptional regulator, at 1,064,076 to 1,064,747
_RS24495
BPHYT_RS24500: BPHYT_RS24500 - histidine kinase, at 1,064,744 to 1,066,144
_RS24500
BPHYT_RS24505: BPHYT_RS24505 - glyoxalase, at 1,066,141 to 1,066,566
_RS24505
BPHYT_RS24510: BPHYT_RS24510 - alkylphosphonate utilization protein, at 1,066,621 to 1,066,962
_RS24510
Position (kb) 1064
1065
1066
1067 Strain fitness (log2 ratio) -1
0
1 at 1063.990 kb on + strand at 1063.990 kb on + strand at 1064.148 kb on + strand, within BPHYT_RS24495 at 1064.726 kb on + strand at 1065.473 kb on + strand, within BPHYT_RS24500 at 1065.587 kb on + strand, within BPHYT_RS24500 at 1066.142 kb on + strand at 1066.230 kb on - strand, within BPHYT_RS24505 at 1066.242 kb on - strand, within BPHYT_RS24505 at 1066.512 kb on - strand, within BPHYT_RS24505 at 1066.586 kb on + strand at 1066.898 kb on + strand, within BPHYT_RS24510 at 1066.898 kb on + strand, within BPHYT_RS24510 at 1066.899 kb on - strand, within BPHYT_RS24510 at 1067.116 kb on + strand at 1067.134 kb on + strand at 1067.135 kb on - strand at 1067.135 kb on - strand at 1067.144 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 1,063,990 + +0.4 1,063,990 + -0.1 1,064,148 + BPHYT_RS24495 0.11 +0.2 1,064,726 + -0.1 1,065,473 + BPHYT_RS24500 0.52 +0.9 1,065,587 + BPHYT_RS24500 0.60 +1.1 1,066,142 + -0.2 1,066,230 - BPHYT_RS24505 0.21 -0.0 1,066,242 - BPHYT_RS24505 0.24 -0.1 1,066,512 - BPHYT_RS24505 0.87 -0.5 1,066,586 + +0.3 1,066,898 + BPHYT_RS24510 0.81 +1.0 1,066,898 + BPHYT_RS24510 0.81 -1.0 1,066,899 - BPHYT_RS24510 0.81 -0.2 1,067,116 + +0.1 1,067,134 + +0.1 1,067,135 - -0.2 1,067,135 - -0.7 1,067,144 + -0.1
Or see this region's nucleotide sequence