Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21995 and BPHYT_RS22000 overlap by 4 nucleotides BPHYT_RS22000 and BPHYT_RS22005 are separated by 23 nucleotides BPHYT_RS22005 and BPHYT_RS22010 are separated by 39 nucleotides
BPHYT_RS21995: BPHYT_RS21995 - (2Fe-2S)-binding protein, at 490,313 to 491,746
_RS21995
BPHYT_RS22000: BPHYT_RS22000 - hypothetical protein, at 491,743 to 492,105
_RS22000
BPHYT_RS22005: BPHYT_RS22005 - ABC transporter permease, at 492,129 to 492,923
_RS22005
BPHYT_RS22010: BPHYT_RS22010 - ABC transporter permease, at 492,963 to 493,877
_RS22010
Position (kb) 491
492
493 Strain fitness (log2 ratio) -1
0
1 at 491.119 kb on - strand, within BPHYT_RS21995 at 491.202 kb on - strand, within BPHYT_RS21995 at 491.232 kb on + strand, within BPHYT_RS21995 at 491.252 kb on + strand, within BPHYT_RS21995 at 491.496 kb on + strand, within BPHYT_RS21995 at 491.788 kb on + strand, within BPHYT_RS22000 at 491.982 kb on + strand, within BPHYT_RS22000 at 492.164 kb on - strand at 492.256 kb on + strand, within BPHYT_RS22005 at 492.256 kb on + strand, within BPHYT_RS22005 at 492.256 kb on + strand, within BPHYT_RS22005 at 492.256 kb on + strand, within BPHYT_RS22005 at 492.262 kb on + strand, within BPHYT_RS22005 at 492.263 kb on - strand, within BPHYT_RS22005 at 492.588 kb on + strand, within BPHYT_RS22005 at 493.006 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 491,119 - BPHYT_RS21995 0.56 -1.7 491,202 - BPHYT_RS21995 0.62 +0.8 491,232 + BPHYT_RS21995 0.64 -0.1 491,252 + BPHYT_RS21995 0.65 -0.2 491,496 + BPHYT_RS21995 0.82 -0.2 491,788 + BPHYT_RS22000 0.12 +1.0 491,982 + BPHYT_RS22000 0.66 -0.5 492,164 - -0.4 492,256 + BPHYT_RS22005 0.16 -0.6 492,256 + BPHYT_RS22005 0.16 -0.1 492,256 + BPHYT_RS22005 0.16 -0.5 492,256 + BPHYT_RS22005 0.16 +0.6 492,262 + BPHYT_RS22005 0.17 -0.9 492,263 - BPHYT_RS22005 0.17 -1.2 492,588 + BPHYT_RS22005 0.58 +0.5 493,006 + +0.4
Or see this region's nucleotide sequence