Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07250 and BPHYT_RS07255 overlap by 4 nucleotides BPHYT_RS07255 and BPHYT_RS07260 overlap by 4 nucleotides BPHYT_RS07260 and BPHYT_RS07265 are separated by 30 nucleotides BPHYT_RS07265 and BPHYT_RS07270 are separated by 14 nucleotides
BPHYT_RS07250: BPHYT_RS07250 - 4-hydroxy-2-oxovalerate aldolase, at 1,646,430 to 1,647,473
_RS07250
BPHYT_RS07255: BPHYT_RS07255 - 4-oxalocrotonate decarboxylase, at 1,647,470 to 1,648,243
_RS07255
BPHYT_RS07260: BPHYT_RS07260 - 2-aminomuconate deaminase, at 1,648,240 to 1,648,671
_RS07260
BPHYT_RS07265: BPHYT_RS07265 - 3-hydroxyanthranilate 3,4-dioxygenase, at 1,648,702 to 1,649,226
_RS07265
BPHYT_RS07270: BPHYT_RS07270 - aminocarboxymuconate-semialdehyde decarboxylase, at 1,649,241 to 1,650,263
_RS07270
Position (kb) 1647
1648
1649 Strain fitness (log2 ratio) -1
0
1
2 at 1646.565 kb on - strand, within BPHYT_RS07250 at 1646.858 kb on + strand, within BPHYT_RS07250 at 1646.926 kb on - strand, within BPHYT_RS07250 at 1647.339 kb on + strand, within BPHYT_RS07250 at 1647.650 kb on - strand, within BPHYT_RS07255 at 1647.669 kb on + strand, within BPHYT_RS07255 at 1647.669 kb on + strand, within BPHYT_RS07255 at 1647.670 kb on - strand, within BPHYT_RS07255 at 1648.230 kb on - strand at 1648.669 kb on + strand at 1649.016 kb on - strand, within BPHYT_RS07265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 1,646,565 - BPHYT_RS07250 0.13 -0.2 1,646,858 + BPHYT_RS07250 0.41 +0.2 1,646,926 - BPHYT_RS07250 0.48 +1.1 1,647,339 + BPHYT_RS07250 0.87 +0.5 1,647,650 - BPHYT_RS07255 0.23 +0.7 1,647,669 + BPHYT_RS07255 0.26 +0.8 1,647,669 + BPHYT_RS07255 0.26 +2.4 1,647,670 - BPHYT_RS07255 0.26 -0.2 1,648,230 - +0.3 1,648,669 + -0.6 1,649,016 - BPHYT_RS07265 0.60 -0.2
Or see this region's nucleotide sequence