Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2014

Experiment: With vitamins t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2013 and Psest_2014 are separated by 191 nucleotidesPsest_2014 and Psest_2015 are separated by 484 nucleotides Psest_2013: Psest_2013 - Predicted phosphatase, at 2,130,695 to 2,132,689 _2013 Psest_2014: Psest_2014 - Isocitrate lyase (EC 4.1.3.1) (from data), at 2,132,881 to 2,134,476 _2014 Psest_2015: Psest_2015 - Type II secretory pathway, component PulD, at 2,134,961 to 2,135,770 _2015 Position (kb) 2132 2133 2134 2135Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2131.890 kb on + strand, within Psest_2013at 2131.890 kb on + strand, within Psest_2013at 2131.890 kb on + strand, within Psest_2013at 2131.890 kb on + strand, within Psest_2013at 2131.890 kb on + strand, within Psest_2013at 2131.890 kb on + strand, within Psest_2013at 2131.890 kb on + strand, within Psest_2013at 2131.890 kb on + strand, within Psest_2013at 2131.891 kb on - strand, within Psest_2013at 2131.891 kb on - strand, within Psest_2013at 2131.891 kb on - strand, within Psest_2013at 2131.891 kb on - strand, within Psest_2013at 2131.891 kb on - strand, within Psest_2013at 2131.891 kb on - strand, within Psest_2013at 2131.891 kb on - strand, within Psest_2013at 2131.963 kb on - strand, within Psest_2013at 2132.135 kb on + strand, within Psest_2013at 2132.135 kb on + strand, within Psest_2013at 2132.136 kb on - strand, within Psest_2013at 2132.136 kb on - strand, within Psest_2013at 2132.199 kb on + strand, within Psest_2013at 2132.200 kb on - strand, within Psest_2013at 2132.200 kb on - strand, within Psest_2013at 2132.359 kb on + strand, within Psest_2013at 2132.362 kb on + strand, within Psest_2013at 2132.362 kb on + strand, within Psest_2013at 2132.362 kb on + strand, within Psest_2013at 2132.431 kb on + strand, within Psest_2013at 2132.516 kb on + strandat 2132.517 kb on - strandat 2132.579 kb on + strandat 2132.579 kb on + strandat 2132.579 kb on + strandat 2132.579 kb on + strandat 2132.580 kb on - strandat 2132.793 kb on + strandat 2132.794 kb on - strandat 2132.910 kb on + strandat 2132.945 kb on + strandat 2133.045 kb on - strand, within Psest_2014at 2133.045 kb on - strand, within Psest_2014at 2133.071 kb on + strand, within Psest_2014at 2133.071 kb on + strand, within Psest_2014at 2133.114 kb on - strand, within Psest_2014at 2133.341 kb on + strand, within Psest_2014at 2133.472 kb on + strand, within Psest_2014at 2133.472 kb on + strand, within Psest_2014at 2133.623 kb on + strand, within Psest_2014at 2133.623 kb on + strand, within Psest_2014at 2133.623 kb on + strand, within Psest_2014at 2133.624 kb on - strand, within Psest_2014at 2133.891 kb on - strand, within Psest_2014at 2133.891 kb on - strand, within Psest_2014at 2133.891 kb on - strand, within Psest_2014at 2133.891 kb on - strand, within Psest_2014at 2133.957 kb on - strand, within Psest_2014at 2134.076 kb on + strand, within Psest_2014at 2134.076 kb on + strand, within Psest_2014at 2134.076 kb on + strand, within Psest_2014at 2134.077 kb on - strand, within Psest_2014at 2134.077 kb on - strand, within Psest_2014at 2134.181 kb on + strand, within Psest_2014at 2134.334 kb on + strandat 2134.424 kb on + strandat 2134.466 kb on + strandat 2134.493 kb on + strandat 2134.493 kb on + strandat 2134.493 kb on + strandat 2134.493 kb on + strandat 2134.530 kb on + strandat 2134.531 kb on - strandat 2134.580 kb on - strandat 2134.783 kb on - strandat 2134.923 kb on + strandat 2135.217 kb on + strand, within Psest_2015at 2135.217 kb on + strand, within Psest_2015at 2135.217 kb on + strand, within Psest_2015at 2135.217 kb on + strand, within Psest_2015at 2135.218 kb on - strand, within Psest_2015at 2135.218 kb on - strand, within Psest_2015at 2135.396 kb on + strand, within Psest_2015at 2135.397 kb on - strand, within Psest_2015

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Per-strain Table

Position Strand Gene LocusTag Fraction With vitamins t1
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2,131,890 + Psest_2013 0.60 +1.6
2,131,890 + Psest_2013 0.60 +0.6
2,131,890 + Psest_2013 0.60 -0.3
2,131,890 + Psest_2013 0.60 -0.2
2,131,890 + Psest_2013 0.60 +0.9
2,131,890 + Psest_2013 0.60 -0.1
2,131,890 + Psest_2013 0.60 +0.9
2,131,890 + Psest_2013 0.60 +0.5
2,131,891 - Psest_2013 0.60 -0.3
2,131,891 - Psest_2013 0.60 +1.2
2,131,891 - Psest_2013 0.60 +1.4
2,131,891 - Psest_2013 0.60 -0.8
2,131,891 - Psest_2013 0.60 +0.4
2,131,891 - Psest_2013 0.60 -0.3
2,131,891 - Psest_2013 0.60 -1.8
2,131,963 - Psest_2013 0.64 -1.3
2,132,135 + Psest_2013 0.72 -1.8
2,132,135 + Psest_2013 0.72 -2.1
2,132,136 - Psest_2013 0.72 +0.2
2,132,136 - Psest_2013 0.72 +1.2
2,132,199 + Psest_2013 0.75 +1.7
2,132,200 - Psest_2013 0.75 -0.1
2,132,200 - Psest_2013 0.75 -1.3
2,132,359 + Psest_2013 0.83 +0.9
2,132,362 + Psest_2013 0.84 -0.6
2,132,362 + Psest_2013 0.84 -0.8
2,132,362 + Psest_2013 0.84 +0.6
2,132,431 + Psest_2013 0.87 +0.2
2,132,516 + -0.5
2,132,517 - -0.0
2,132,579 + -0.6
2,132,579 + -1.9
2,132,579 + +0.6
2,132,579 + +3.2
2,132,580 - +0.9
2,132,793 + -2.1
2,132,794 - +0.5
2,132,910 + -2.6
2,132,945 + -0.1
2,133,045 - Psest_2014 0.10 -0.5
2,133,045 - Psest_2014 0.10 -3.2
2,133,071 + Psest_2014 0.12 -0.3
2,133,071 + Psest_2014 0.12 -1.3
2,133,114 - Psest_2014 0.15 -0.1
2,133,341 + Psest_2014 0.29 -0.2
2,133,472 + Psest_2014 0.37 +0.5
2,133,472 + Psest_2014 0.37 +2.2
2,133,623 + Psest_2014 0.46 -1.9
2,133,623 + Psest_2014 0.46 -2.6
2,133,623 + Psest_2014 0.46 -0.2
2,133,624 - Psest_2014 0.47 -0.7
2,133,891 - Psest_2014 0.63 -1.1
2,133,891 - Psest_2014 0.63 +1.9
2,133,891 - Psest_2014 0.63 -1.8
2,133,891 - Psest_2014 0.63 +0.2
2,133,957 - Psest_2014 0.67 -0.3
2,134,076 + Psest_2014 0.75 -1.3
2,134,076 + Psest_2014 0.75 -0.7
2,134,076 + Psest_2014 0.75 -2.2
2,134,077 - Psest_2014 0.75 -1.6
2,134,077 - Psest_2014 0.75 -0.4
2,134,181 + Psest_2014 0.81 +1.0
2,134,334 + +0.2
2,134,424 + -1.3
2,134,466 + -0.8
2,134,493 + +0.3
2,134,493 + +0.2
2,134,493 + -1.1
2,134,493 + -0.4
2,134,530 + -1.8
2,134,531 - -1.8
2,134,580 - +1.6
2,134,783 - -0.1
2,134,923 + +0.2
2,135,217 + Psest_2015 0.32 -1.4
2,135,217 + Psest_2015 0.32 -1.0
2,135,217 + Psest_2015 0.32 +0.5
2,135,217 + Psest_2015 0.32 +2.3
2,135,218 - Psest_2015 0.32 -0.4
2,135,218 - Psest_2015 0.32 +0.8
2,135,396 + Psest_2015 0.54 +0.2
2,135,397 - Psest_2015 0.54 -1.4

Or see this region's nucleotide sequence